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Joint expression of Zmpepc, Zmppdk, and Zmnadp-me is more efficient than expression of one or two of those genes in improving the photosynthesis of Arabidopsis

文献类型： 外文期刊

作者： Zhang, Qingchen 1 ; Li, Yan 2 ; Xu, Weigang 2 ; Zhang, Yu 2 ; Qi, Xueli 2 ; Fang, Yuhui 2 ; Peng, Chaojun 2 ;

作者机构： 1.Henan Agr Univ, Coll Agron, Zhengzhou 450002, Peoples R China

2.Henan Acad Agr Sci, Inst Crop Mol Breeding, Key Lab Wheat Biol & Genet Breeding Cent Huanghua, Natl Engn Lab Wheat,Minist Agr, Zhengzhou 450002, Peoples R China

3.Shangqiu Normal Univ, Coll Biol & Food, Shangqiu 476000, Peoples R China

关键词： Arabidopsis; Chlorophyll a fluorescence; Chloroplast proteomics; Phosphoenolpyruvate carboxylase (PEPC); Pyruvate phosphate dikinase (PPDK); NADP-Malic enzyme (NADP-ME)

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2021 年 158 卷

页码：

收录情况： SCI

摘要： This study assessed the effects of seven combinations of maize (Zea mays) genes phosphoenolpyruvate carboxylase (pepc), pyruvate phosphate dikinase (ppdk), and NADP-malic enzyme (nadp-me), on the photosynthesis of Arabidopsis. The photosynthetic rate, carboxylation efficiency, and shoot-dry-weight of Zmpepc (PC), Zmpepc + Zmppdk (PCK), Zmpepc + Zmnadp-me (PCM), and Zmpepc + Zmppdk + Zmnadp-me (PCKM) were significantly higher than those of the control wild-type (WT), with a trends to be PCKM > PCK > PC and PCM > WT. This indicated that Zmpepc was a prerequisite for improved photosynthetic performance; Zmppdk had a positive effect on Zmpepc, and the triple gene combination had the most significant synergistic effects. PCKM significantly enhanced activity of photosystem (PS)II (K, J phase) and PSI, light energy absorption (ABS/CSm) and conversion (TRo/ABS), and electron transfer (ETo/TRo). PCKM up-regulated 18 photosynthesis-related proteins, among which, 11 were involved in light reaction resulting in improved light-energy absorption and conversion efficiency, electron transfer, activity and stability of PSII and PSI, and the ATP and NADPH production. The remaining seven proteins were involved in dark reaction. The up-regulation of these proteins in PCKM improved the coordinated operation of light and dark reaction, increasing the photosynthesis and dry weight ultimately. These results also provide a promising strategy for the genetic improvement of the photosynthetic performance of C-3 crops by inserting major C-4 photosynthetic genes.

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