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A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer

文献类型: 外文期刊

作者: Li, Xuefei 1 ; Liu, Qingcheng 2 ; Li, Shiyu 1 ; Sossah, Frederick Leo 1 ; Han, Xuerong 1 ; Zhu, Guosheng 2 ; Li, Yu 1 ; Li, Changtian 1 ; Fu, Yongping 1 ;

作者机构: 1.Jilin Agr Univ, Chinese Minist Educ Edible & Med Fungi, Engn Res Ctr, Changchun 130118, Jilin, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Key Lab Edible Fungi Breeding, Guiyang 550006, Guizhou, Peoples R China

3.Guizhou Acad Agr Sci, Crop Germplasm Resources, Guiyang 550006, Guizhou, Peoples R China

4.Council Sci & Ind Res CSIR, Oil Palm Res Inst, Coconut Res Programme, POB 245, Sekondi Takoradi, Ghana

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.685; 五年影响因子:2.506 )

ISSN: 0304-8608

年卷期: 2022 年 167 卷 11 期

页码:

收录情况: SCI

摘要: Here, we report the characterization of a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq, reverse transcription polymerase chain reaction, 5 ' and 3 ' rapid amplification of cDNA ends, and Sanger sequencing. Based on database searches, sequence alignment, and phylogenetic analysis, we designated the virus as "Auricularia heimuer negative-stranded RNA virus 1" (AhNsRV1). This virus has a monopartite RNA genome related to mymonaviruses (order Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and contains six open reading frames (ORFs). The largest ORF encodes a putative RNA-dependent RNA polymerase; the other ORFs encode hypothetical proteins with no conserved domains or known function. AhNsRV1 is the first (-)ssRNA virus and the third virus known to infect A. heimuer.

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