A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer
文献类型： 外文期刊
作者： Li, Xuefei 1 ; Liu, Qingcheng 2 ; Li, Shiyu 1 ; Sossah, Frederick Leo 1 ; Han, Xuerong 1 ; Zhu, Guosheng 2 ; Li, Yu 1 ; Li, Changtian 1 ; Fu, Yongping 1 ;
作者机构： 1.Jilin Agr Univ, Chinese Minist Educ Edible & Med Fungi, Engn Res Ctr, Changchun 130118, Jilin, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Key Lab Edible Fungi Breeding, Guiyang 550006, Guizhou, Peoples R China
3.Guizhou Acad Agr Sci, Crop Germplasm Resources, Guiyang 550006, Guizhou, Peoples R China
4.Council Sci & Ind Res CSIR, Oil Palm Res Inst, Coconut Res Programme, POB 245, Sekondi Takoradi, Ghana
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.685； 五年影响因子：2.506 ）
ISSN： 0304-8608
年卷期： 2022 年 167 卷 11 期
页码：
收录情况： SCI
摘要： Here, we report the characterization of a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq, reverse transcription polymerase chain reaction, 5 ' and 3 ' rapid amplification of cDNA ends, and Sanger sequencing. Based on database searches, sequence alignment, and phylogenetic analysis, we designated the virus as "Auricularia heimuer negative-stranded RNA virus 1" (AhNsRV1). This virus has a monopartite RNA genome related to mymonaviruses (order Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and contains six open reading frames (ORFs). The largest ORF encodes a putative RNA-dependent RNA polymerase; the other ORFs encode hypothetical proteins with no conserved domains or known function. AhNsRV1 is the first (-)ssRNA virus and the third virus known to infect A. heimuer.
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