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Nucleoside Diphosphate Kinases (ndk) reveals a key role in adhesion and virulence of Aeromonas veronii

文献类型： 外文期刊

作者： Zhang, Lei 1 ; Song, Mingfang 1 ; Yang, Nuo 2 ; Zhang, XiuWen 3 ; Raza, Sayed Haidar Abbas 5 ; Jia, Kaixiang 1 ; Tian, 1 ;

作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Prod & Prod Qual Safety, Minist Educ, Changchun 130118, Peoples R China

2.First Hosp Jilin Univ, Dept Pediat Neurol, Changchun 130021, Jilin, Peoples R China

3.Jilin Acad Agr Sci, Res Management Off, Changchun 130033, Peoples R China

4.Hebei Normal Univ Sci & Technol, Coll Anim Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China

5.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shanxi, Peoples R China

关键词： Aeromonas veronii; ndk gene; Virulence; Biofilm formation

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2020 年 149 卷

页码：

收录情况： SCI

摘要： Aeromous veronii is a severe pathogen that can infect aquatic organisms and mammals also causes irreparable damage to fish aquaculture. Analysis of the results of epidemiological investigations have revealed that its tolerance to drugs and the virulence of A. veronii have increased in recent years. Most of the researches on A. veronii focuse on the strain isolation, identification, and drug susceptibility. However, we do not know so much about the molecular mechanism of the pathogenesis on A. veronii. Here we identified and obtained the highly expressed TH0426 Nucleoside Diphosphate Kinases (NDK) of A. veronii. We first constructed a mutant strain (Delta-ndk) by generating an in-frame deletion of the ndk gene, to investigate the functional role in A. veronii TH0426. The ability in the adhesion and invasion of EPC cells and biofilm formation significantly reduced of the Delta-ndk strain. The motility test showed that the ndk gene affected on the swimming ability, while did not affect the swarming motility. Compared with the wild-type strain TH0426, the pathogenicity of Delta-ndk strain to zebrafish reduced severely. Besides, the ndk gene has affected the apoptosis rate of A. veronii TH0426. These results would help to demonstrate the function of ndk further and realize the pathogenesis on A. veronii.

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