文献类型： 外文期刊

作者： Wang, Weilong ¹ ; Lian, Qingping ³ ; Chen, Yuange ⁴ ; Hiramatsu, Naoshi ⁵ ; Wu, Meiqin ⁶ ;

作者机构： 1.Shanghai Ocean Univ, Minist Agr, Ctr Res Environm Ecol & Fish Nutr, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China

3.Zhejiang Inst Freshwater Fisheries, Agr Minist, Key Lab Hlth Freshwater Aquaculture, Key Lab Freshwater Aquaculture Genet & Breeding Z, Huzhou 313001, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

5.Hokkaido Univ, Fac Fisheries Sci, Div Marine Life Sci, Hakodate, Hokkaido 0418611, Japan

6.Shanghai Ocean Univ, Coll Marine Ecol & Environm, Shanghai 201306, Peoples R China

关键词： Vitellogenin; Recombinant protein; Polyclonal antibodies; Misgurnus anguillicaudatus; 17 alpha-ethinylestradiol; 17 beta-estradiol

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2021 年 532 卷

页码：

收录情况： SCI

摘要： Fish vitellogenin (Vtg) has become an important biomarker for assessing the estrogenic potency of chemicals and the exposure of animals to estrogenic contaminants present in aquatic environments. In the current study, the polyclonal antibodies against subtype-specific Vtgs of the dojo loach (Misgurnus anguillicaudatus) were developed and identified by gene recombination and expression. Immuno-biochemical analyses revealed that VtgAo1 protein appeared to be the major Vtg type in this species. Enhanced chemiluminescent Western blotting was developed using the antiserum against VtgAo1. Exposure of male loach to 17 alpha-ethinylestradiol (EE2) and 17 beta-estradiol (E2) via water induced the VtgAo1 protein induction with a lowest-observed-effect concentration (LOEC) by 100 ng L-1 and 1000 ng L(-1)group at 7 day post-initiation (dpi), respectively. The results indicated that this method provided a valuable tool for detecting estrogenic activities in aquatic environments and excluded any difficulties expected in purification procedures to separate a highly pure Vtg subtype from circulating proteins, and the other Vtg subtypes.