TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat
文献类型： 外文期刊
作者： Miao Li-li 1 ; Li Yu-ying 3 ; Zhang Hong-juan 3 ; Zhang Hong-ji 2 ; Liu Xiu-lin 4 ; Wang Jing-yi 3 ; Chang Xiao-ping 3 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Postdoctoral Programme, Harbin 150086, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Crop Resources, Harbin 150086, Peoples R China
3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
4.Heilongjiang Acad Agr Sci, Inst Soybean, Harbin 150086, Peoples R China
关键词： TaSnRK2.4; association analysis; TKW; abiotic stress
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2021 年 20 卷 1 期
页码：
收录情况： SCI
摘要： Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli. Previous studies showed that TaSnRK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis. Further experiments were performed to decipher the underlying mechanisms and discover new functions. The genomic sequences of TaSnRK2.4s locating on chromosome 3A, 3B and 3D were obtained. Sequencing identified one and 13 variations of TaSnRK2.4-3A and TaSnRK2.4-3B, respectively, but no variation was detected in TaSnRK2.4-3D. The markers 2.4AM1, 2.4BM1 and 2.4BM2 were developed based on three variations. Association analysis showed that both TaSnRK2.4-3A and TaSnRK2.4-3B were significantly associated with thousand-kernel weight (TKW), and that SNP3A-T and SNP3B-C were favorable alleles for higher TKW. Yeast two-hybrid and split luciferase assays showed that TaSnRK2.4 physically interacted with abiotic stress responsive protein TaLTP3, suggesting that TaSnRK2.4 enhanced abiotic stress tolerance by activating TaLTP3. Our studies suggested that TaSnRK2.4 have potential in improving TKW and response to abiotic stress.
- 相关文献
