文献类型： 外文期刊

作者： Liu, S. ¹ ; Liu, M. ¹ ; Liao, Q-G ¹ ; Lu, F-B ⁵ ; Zhao, X-L ¹ ;

作者机构： 1.South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat Forest P, 483 Wushan Rd, Guangzhou 510642, Peoples R China

2.Huizhou Univ, Huizhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Crop Res Inst, Guangzhou, Peoples R China

4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen, Guangdong, Peoples R China

5.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangzhou, Peoples R China

关键词： Cymbidium hybridum; endophytic fungi; fungal community composition; nested PCR nutrient uptake; orchid mycorrhiza

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.772； 五年影响因子：3.963 ）

ISSN： 1364-5072

年卷期：

页码：

收录情况： SCI

摘要： Aims The aim of this study was to assess the effects of beneficial micro-organisms on the growth, nutrient accumulation and root-associated fungal species composition of pot orchids grown in the greenhouse. Methods and Results A greenhouse pot experiment was conducted to investigate the beneficial effects of a mycorrhizal fungus, Epulorhiza repens isolate ML01, an endophytic fungus, Umbelopsis nana isolate ZH3A-3 and a mixed commercial inoculum Rem, alone or in combination. Nested PCR assays showed that both isolates ML01 and ZH3A-3 can successfully establish in inoculated soil. All the inoculants significantly increased the plant total dry weight of Cymbidium hybridum 'Golden Boy', whereas only co-inoculation with the endophytic fungus ZH3A-3 and the Rem enhanced the fresh weight and height of host plants. The mycorrhizal fungus positively affected P, K, Ca, Mg content in shoots and Zn content in roots, while the endophytic fungus improved N, P, Ca accumulation in shoots and roots. Co-inoculation with the Rem and ML01 improved root to shoot translocation of Fe and Zn. In addition, inoculation with ZH3A-3, ML01+Rem and ZH3A-3+Rem decreased the relative frequency of Fusarium sp. on orchid roots. Trichoderma sp. were isolated from the roots treated with ML01, ML01+Rem and ZH3A-3+Rem. Conclusions Both mycorrhizal and endophytic fungi had the potential to create favourable microflora in the orchid roots and stimulate the growth of transplanted plantlets under greenhouse condition. Significance and Impact of the Study The newly isolated endophytic strain ZH3A-3 showed significant application value in orchid production.