Alkaline phosphatase can promote the replication of Bombyx mori cypovirus 1 by interaction with its turret protein
文献类型： 外文期刊
作者： Lu, Qiuyuan 1 ; Ren, Feifei 1 ; Yan, Jiming 1 ; Zhang, Yinong 1 ; Awais, Mian 1 ; He, Jian 3 ; Sun, Jingchen 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Subtrop Sericulture & Mulberry Resources Protect, Guangzhou 510642, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Publ Expt Teaching Ctr, Guangzhou 510642, Guangdong, Peoples R China
关键词： Alkaline phosphatase; Bombyx mori; BmCPV1; Turret protein; Replication; RNA triphosphatase
期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）
ISSN： 0168-1702
年卷期： 2021 年 292 卷
页码：
收录情况： SCI
摘要： Bombyx mori cypovirus 1 (BmCPV1) is a member of the Reoviridae family which is characterized by its singlelayered capsid. Similar with other turreted viruses in the Reoviridae, transcription of BmCPV1 occurs inside the capsid, and the nascent mRNA is released to the turret which consists of five turret proteins (TPs) and located at the 5-fold axis of the outer capsid, then the capping enzyme TP will guanylate and methylate the nascent viral mRNA to produce a matured mRNA. However, during these processes, how the BmCPV1 draws other cellular proteins to facilitate its replication is still lesser-known. Here we used an ELISA to investigate the interaction between ALP and BmCPV1. A co-immunoprecipitation technique was employed to detect the interaction of ALP with the Methylase domain of TP. We further studied whether ALP affects the replication of BmCPV1 inside the cell, results show that reducing the expression of ALP through RNAi reduced the transcription level of the BmCPV1 VP1 gene, which was increased by overexpression of ALP. In summary, our data demonstrate an interaction between ALP and BmCPV1 and that ALP promoted the replication of BmCPV1, and support our hypothesis of the ALP is an RTPase to facilitate the capping process of BmCPV1.
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