文献类型： 外文期刊

作者： Tan, Yanping ¹ ; Yang, Xiaolin ² ; Pei, Minghao ¹ ; Xu, Xin ¹ ; Wang, Chuntai ¹ ; Liu, Xinqiong ¹ ;

作者机构： 1.South Cent Univ Nationalities, Coll Life Sci, Key Lab State Ethn Affairs Commiss Biol Technol, Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Peoples R China

2.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Hubei Key Lab Crop Dis Insect Pests & Weeds, Wuhan, Peoples R China

关键词： Rice; Magnaporthe oryzae; microarray; interaction

期刊名称：BIOENGINEERED （ 影响因子：3.269； 五年影响因子：2.916 ）

ISSN： 2165-5979

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： SCI

摘要： The main aim of the work is to study the regulation of gene expression in the interaction between rice and Magnaporthe oryzae by gene chip technology. In this study, we mainly focused on changes of gene expression at 24, 48, and 72 hours post-inoculation (hpi), through which we could conduct a more comprehensive analysis of rice blast-related genes in the process of infection. The results showed that the experimental groups contained 460, 1227, and 3937 significant differentially expressed genes at 24, 48, and 72 hpi, respectively. Furthermore, 115 significantly differentially expressed genes were identified in response to rice blast infection at all three time points. By annotating these 115 genes, they were divided into three categories: metabolic pathways, proteins or enzymes, and organelle components. As expected, many of these genes were known rice blast-related genes; however, we discovered new genes with high fold changes. Most of them encoded conserved hypothetical proteins, and some were hypothetically conserved genes. Our study may contribute to finding new resistance genes and understanding the mechanism of rice blast development.