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Reference genes for the study of herbicide stress responses in Leptochloa chinensis (L.) Nees and estimation of ACCase expression in cyhalofop-butyl resistant populations

文献类型： 外文期刊

作者： Zhang, Yi 1 ; Chen, Liping 1 ; Song, Wen 1 ; Cang, Tao 1 ; Xu, Mingfei 1 ; Zhou, Guojun 2 ; Wu, Changxing 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, MOA Key Lab Pesticide Residue Detect, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Peoples R China

2.Shaoxing Acad Agr Sci, Shaoxing 312003, Peoples R China

关键词： Leptochloa chinensis; Cyhalofop-butyl; Herbicide resistance; Quantitative PCR; Reference genes; ACCase

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2021 年 171 卷

页码：

收录情况： SCI

摘要： Cyhalofop-butyl resistance in Leptochloa chinensis (L.) Nees is a threat to rice production. Qualitative changes to the acetyl-CoA carboxylase gene (ACCase) have been reported to induce cyhalofop-butyl resistance in some weed species, but the role of ACCase in cyhalofop-butyl resistance through quantitative changes remains uncertain. The accurate assessment of transcriptional changes in the functional genes associated with herbicide resistance in L. chinensis is challenging owing to the lack of available reference genes for expression normalization. Here, we selected nine candidate reference genes in L. chinensis and assessed their transcription stability in populations susceptible and resistant to cyhalofop-butyl. Transcription stability was compared under conditions of herbicide stress and control conditions using BestKeeper, NormFinder, and geNorm. Elongation factor 1 alpha, eukaryotic initiation factor 4A, and cap-binding protein CBP20 were the most stable reference genes under cyhalofop-butyl treatment. Transcription levels of ACCase were evaluated in seven resistant populations, one of which showed higher transcription than the susceptible population after 24 h cyhalofop-butyl treatment. However, the slight up-regulation of ACCase (approximately 2.0-fold) is unlikely to be responsible for the high resistance levels in these populations of L. chinensis.

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