您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain

文献类型： 外文期刊

作者： Lin, Weiling 1 ; Xu, Xiaohong 3 ; Lv, Ruirui 1 ; Huang, Wei 1 ; ul Haq, Hafeez 1 ; Gao, Yuanyuan 1 ; Ren, Hongli 1 ; Lan, 1 ;

作者机构： 1.Fujian Normal Univ, Coll Life Sci, Engn Res Ctr Ind Microbiol Minist Educ, 8 Shangsan Rd, Fuzhou 350108, Fujian, Peoples R China

2.Fujian Hlth Coll, Fuzhou 350101, Fujian, Peoples R China

3.Fujian Normal Univ, Lib, Fuzhou 350108, Fujian, Peoples R China

4.Fujian Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Fuzhou 350003, Fujian, Peoples R China

关键词： Aspergillus niger mutant; Differential proteomics; Key determinants; Pectin-degrading enzymes; Synergy

期刊名称：BIOTECHNOLOGY LETTERS （ 2020影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2021 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Objectives To reveal the potential mechanism and key determinants that contributed to the improved pectinase activity in Aspergillus niger mutant EIMU2, which was previously obtained by UV-mutagenesis from the wild-type A. niger EIM-6. Results Proteomic analysis for Aspergillus niger EIMU2 by two-dimensional electrophoresis demonstrated that mutant EIMU2 harbored a multiple enzyme system for the degradation of pectin, mainly constituting by main-chain-cleaving enzymes polygalacturonase, pectate lyase, pectinesterase, and some accessory enzymes rhamnogalacturonan lyase and arabinofuranosidase. Further quantitatively differential proteomic analysis revealed that the quantities of four proteins, pectinesterase, rhamnogalacturonan lyase A, DNA-directed RNA polymerase A, and a hypothetical protein in strain EIMU2 were much higher than those in EIM-6. PCR amplification, sequencing and alignment analysis of genes for the two main members of pectin-degrading enzymes, pectate lyase and polygalacturonase showed that their sequences were completely consistent in A. niger EIM-6 and mutant EIMU2. Conclusions The result demonstrated that the improved pectinase activity by UV-mutagenesis in A. niger EIMU2 was probably contributed to the up-regulated expression of rhamnogalacturonan lyase, or pectinesterase, which resulted in the optimization of synergy amongst different components of pectin-degrading enzymes.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台