An optimized procedure for detection of genetically modified DNA in refined vegetable oils
文献类型： 外文期刊
作者： Duan, Yuzhu 1 ; Pi, Yan 1 ; Li, Changwen 2 ; Jiang, Keji 2 ;
作者机构： 1.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
3.Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： Refined vegetable oil; PCR; Enrichment treatment; Genetically modified oil detection; DNA isolation
期刊名称：FOOD SCIENCE AND BIOTECHNOLOGY （ 影响因子：2.391； 五年影响因子：2.108 ）
ISSN： 1226-7708
年卷期： 2021 年 30 卷 1 期
页码：
收录情况： SCI
摘要： In this study, the amplifiable DNA from refined vegetable oils was isolated by using commercial DNA extraction kits based on the CTAB method in combination with nucleic acid enrichment, and then the presence of genetically modified (GM) soybean and maize DNA in the oils was traced by PCR. The results showed that the duration and intensity of heating had no significant effect on the DNA stability and concentration in oils for a short period, suggesting that DNA in oils could be stably reserved for a certain time, thus making it possible to trace down refined vegetable oils reliably and effectively. The results provided a set of primers suitable for systematic GM oil detection. More importantly, this study made an important contribution to the economical and reliable detection of GM vegetable oils regarding food authenticity issues.
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