文献类型： 外文期刊
作者： Cheng, Lina 1 ; Zhu, Zhiwei 1 ; Sun, Da-Wen 1 ;
作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Peoples R China
2.South China Univ Technol, Guangzhou Higher Educ Mega Ctr, Acad Contemporary Food Engn, Guangzhou 510006, Peoples R China
3.Guangzhou Higher Educ Mega Ctr, Engn & Technol Res Ctr Guangdong Prov Intelligent, Guangzhou 510006, Peoples R China
4.Guangzhou Higher Educ Mega Ctr, Guangdong Prov Engn Lab Intelligent Cold Chain Lo, Guangzhou 510006, Peoples R China
5.Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Key Lab Funct Foods, Minist Agr & Rural Affairs,Guangdong Key Lab Agr, Guangzhou 510610, Peoples R China
6.Natl Univ Ireland, Univ Coll Dublin, Agr & Food Sci Ctr, Food Refrigerat & Computerized Food Technol FRCFT, Dublin 4, Ireland
关键词： High pressure freezing; Thermodynamics; Conformation; PPO; Denaturation
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2021 年 335 卷
页码：
收录情况： SCI
摘要： The mechanism of enzyme protein denaturation induced by high pressure freezing is complicated and unclear as this process involves Pressure-Factors (pressure and time) and Freezing-Factors (temperature, phase transition, recrystallization, and ice crystal types). In this study, the thermodynamics and conformation changes of mushroom polyphenol oxidase (PPO) under high pressure freezing treatments (HPF, P-100,150,200,300,400,500MPa(20 degrees C/30min)) and high pressure processes (HPP) followed with normal pressure immersion freezing (HPP-IF, P-100500MPa(25 degrees C/30min) - P-0.1MPa(-20 degrees C/30min)) are investigated as compared with that processed under high pressure processes (HPP, P-100-500MPa(25 degrees C/30min)) and normal pressure immersion freezing process (IF, P-0.1MPa(-20 degrees C/30min)). The results suggested that the treated PPO with the same enzyme activity may have various thermodynamic characteristics and conformations; Pressure-Factors play the main roles in the denaturation of the PPO during the HPF treatment, and Freezing-Factors can weak the effect of Pressure-Factors on PPO denaturation; The treated PPO may be transferred into a partially fold intermediate state.
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