您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Fine Mapping of a Locus Underlying the Ectopic Blade-Like Outgrowths on Leaf and Screening Its Candidate Genes in Rapeseed (Brassica napus L.)

文献类型： 外文期刊

作者： Chai, Liang 2 ; Feng, Bin 4 ; Liu, Xun 1 ; Jiang, Liangcai 2 ; Yuan, Shu 5 ; Zhang, Zhongwei 5 ; Li, Haojie 2 ; Zhang, Ji 1 ;

作者机构： 1.Guizhou Educ Univ, Sch Biol Sci, Guiyang, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu, Peoples R China

3.Guizhou Acad Agr Sci, Rape Res Inst, Guiyang, Peoples R China

4.Guizhou Acad Agr Sci, Guizhou Oil Res Inst, Guiyang, Peoples R China

5.Sichuan Agr Univ, Coll Resources, Chengdu, Peoples R China

6.Univ Manitoba, Dept Plant Sci, Winnipeg, MB, Canada

关键词： aberrant leaf; Brassica napus; LATE MERISTEM IDENTITY1; REDUCED COMPLEXITY; fine mapping; whole genome re-sequencing

期刊名称：FRONTIERS IN PLANT SCIENCE （ 2020影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 11 卷

页码：

收录情况： SCI

摘要： Leaf is an important organ for higher plants, and the shape of it is one of the crucial traits of crops. In this study, we investigated a unique aberrant leaf morphology trait in a mutational rapeseed material, which displayed ectopic blade-like outgrowths on the adaxial side of leaf. The abnormal line 132000B-3 was crossed with the normal line 827-3. Based on the F-2(:)3 family, we constructed two DNA pools (normal pool and abnormal pool) by the bulked segregant analysis (BSA) method and performed whole genome re-sequencing (WGR), obtaining the single-nucleotide polymorphism (SNP) and insertion/deletion (InDel) data. The SNP-index method was used to calculate the Delta(SNP/InDel-index), and then an association region was identified on chromosome A10 with a length of 5.5 Mbp, harboring 1048 genes totally. Subsequently, the fine mapping was conducted by using the penta-primer amplification refractory mutation system (PARMS), and the associated region was narrowed down to a 35.1-kbp segment, containing only seven genes. These seven genes were then analyzed according to their annotations and finally, BnA10g0422620 and BnA10g0422610, orthologs of LATE MERISTEM IDENTITY1 (LMI1) gene from Arabidopsis and REDUCED COMPLEXITY (RCO) gene from its relative Cardamine hirsuta, respectively, were identified as the candidate genes responding to this blade-like outgrowth trait in rapeseed. This study provides a novel perspective into the leaf formation in Brassica plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台