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HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line

文献类型： 外文期刊

作者： Sun, Suling 1 ; Wang, Wei 1 ; Wang, Nan 2 ; Zhang, Yu 1 ; Zhu, Zuoyi 1 ; Li, Xue 1 ; Wang, Junhong 1 ; Chen, Qihe 3 ; Sadiq, 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Peoples R China

2.Zhejiang Shuren Univ, Coll Biol & Environm Engn, Hangzhou 310015, Peoples R China

3.Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310058, Peoples R China

4.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

5.Shanxi Univ, Sch Life Sci, Taiyuan 030006, Peoples R China

关键词： His-Pro-Pro; Ser-Gly-Gln-Arg; Silkworm pupae; 3-Hydroxy-3-methyl glutaryl coenzyme A reductase; Molecular docking

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：4.451； 五年影响因子：4.907 ）

ISSN： 1756-4646

年卷期： 2021 年 77 卷

页码：

收录情况： SCI

摘要： To screen several peptides that could possibly inhibit the biosynthesis of cholesterol, we selected peptides with high 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) inhibitory activity from silkworm pupae protein hydrolysates (SPPHs). SPPH was obtained by hydrolysing the proteins with neutral proteinase, and filtered to <3, 3-5 and >5 kDa fractions. Fraction 4 (SPP) with the highest HMGCR inhibitory activity was acquired from the ultrafiltrate (<3 kDa) by Sephadex G-15 filtration. In silico predictions were executed to identify peptides with HMGCR inhibitory activity. In vitro studies showed that His-Pro-Pro (HPP) and Ser-Gly-Gln-Arg (SGQR) inhibited activity of HMGCR, decreased mRNA and protein expression of HMGCR and squalene synthase, and activated the expression of low-density lipoprotein receptor. Molecular docking revealed that H-bonding interactions were responsible in HPP and SGQR for their role in improving hypercholesterolemia. Our findings suggest that HPP and SGQR have great potential in improving hypercholesterolemia.

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