Acinetobacter indicus Coharboring Tet (X6) and bla NDM-1 Isolated From Slaughterhouse Waste
文献类型： 外文期刊
作者： Long, Xiaoqian 1 ; Lin, Feng 4 ; Tang, Biao 5 ; Miao, Fengcheng 3 ; Li, Zhiyu 3 ; Shen, Yao 3 ; Yang, Hua 1 ; Ma, Jiangang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou, Zhejiang, Peoples R China
3.Xianghu Lab, Hangzhou, Peoples R China
4.Minist Agr & Rural Affairs, Zhejiang Inst Freshwater Fisheries, Key Lab Hlth Freshwater Aquaculture, Key Lab Fish Hlth & Nutr Zhejiang Prov, Huzhou, Zhejiang, Peoples R China
5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou, Peoples R China
关键词： Acinetobacter indicus; Tet (X6); Plasmid-mediated; Bla NDM-1
期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：3.2； 五年影响因子：3.8 ）
ISSN： 2213-7165
年卷期： 2025 年 41 卷
页码：
收录情况： SCI
摘要： Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment. Methods: A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of bla NDM-1 and tet (X6). Results: The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring bla NDM-1 and tet (X6). The bla NDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, bla NDM-1 is located on the transposon Tn125, and bla NDM-1 can be transferred to other species with the help of transposon. The genetic background of bla NDM-1 is "ISAba125-blaNDM-1-bleMEL-dsbD-cutA- groES- groEL- insE-ISAba125 ". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet (X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene estT, and there is a single copy of the insertion element ISCR2 around tet (X6) as the genetic background"ISAba4-IS3-hp-hp- tet (X6)- estT- guaA-ISCR2 ". Conclusions: This is the first report of the coexistence of tet (X6) and bla NDM-1 in the A. indicus, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the "One Heath". (c) 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
- 相关文献
作者其他论文 更多>>
-
The Effect of Clostridium butyricum-Derived Lipoteichoic Acid on Lipopolysaccharide-Stimulated Porcine Intestinal Epithelial Cells
作者：Chen, Qu;Ma, Lingyan;Wen, Yang;Lyu, Wentao;Yu, Minjie;Yang, Hua;Xiao, Yingping
关键词：
Clostridium butyricum ; inflammatory factors; IPEC-J2; lipopolysaccharide; lipoteichoic acid -
Genome-based assessment of antimicrobial resistance of Escherichia coli recovered from diseased swine in eastern China for a 12-year period
作者：Li, Junxing;Yuan, Xiufang;Xu, Lihua;Yu, Bin;Su, Fei;Ye, Shiyi;Chen, Yijie;Chang, Jiang;Wu, Jing;Guan, Chunjiu;Tang, Biao;Chang, Jiang;Yang, Yue;Zhao, Guoping;Tang, Biao;Ma, Jiangang;Zhou, Wei;Zhao, Guoping;Zhao, Guoping
关键词：
Escherichia coli ; swine; antimicrobial resistance;mcr ;tet (X4) -
In-situ supramolecular self-assembly strategy to fabricate carbon-doped g-C3N4 microtubes: Efficient photocatalytic removal of antibiotics and bacterial inactivation
作者：Lei, Hua;Hu, Ruibin;Yang, Hua;Lei, Hua;Lin, Jianhan;Bian, Changhao;Guo, Fengyu;Dong, Xiaoping;Guo, Fengyu;Xiao, Xingning;Yang, Hua;Wang, Wen
关键词：Supramolecular self-assembly strategy; Visible light catalysis; Tetracycline hydrochloride degradation; Bacterial inactivation; Supramolecular self-assembly strategy; Carbon-dopped g-C3N4; Visible light catalysis; Tetracycline hydrochloride degradation; Bacterial inactivation
-
Novel microfluidic chips integrated with smart devices for in situ detection of foodborne pathogenic bacteria
作者：Hu, Ruibin;Zheng, Qingfeng;Lin, Jianhan;Yang, Hua;Wang, Wen;Wang, Wen;Lin, Jianhan;Yang, Hua
关键词：
-
AMPGen: an evolutionary information-reserved and diffusion-driven generative model for de novo design of antimicrobial peptides
作者：Jin, Shuwen;Zeng, Zihan;Xiong, Xiyan;Huang, Baicheng;Wang, Hongsheng;Ma, Xiao;Zeng, Zihan;Tang, Li;Tang, Xiaochun;Shao, Guoqing;Lin, Feng;Huang, Xingxu;Lin, Feng
关键词：
-
Integrated 16S rRNA and metagenomic sequencing reveals the distribution of key antibiotic resistance genes in duck gut microbiota
作者：Zeng, Hongbo;Yang, Hua;Ma, Lingyan;Lu, Lizhi;Zeng, Tao;Xiao, Yingping;Lyu, Wentao;Fu, Zixian
关键词：Antibiotic resistance gene; 16S rRNA gene sequencing; Metagenomic analysis; Gut microbiota; Duck
-
Integrated Metabolome and Microbiome Analysis Reveals the Regulatory Effects of Fermented Soybean Meal on the Gut Microbiota of Late Gestation
作者：Li, Yantao;Ren, Ying;Li, Yantao;Fu, Lele;Chen, Yushi;Yang, Hua;Wang, Cheng;Yang, Hua;Xiao, Yingping
关键词：gut microbiota; metabolite; fermentation; soybean meal; late gestation
