A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis
文献类型： 外文期刊
作者： Wang, L. 1 ; Chen, H. 1 ; Qiu, L. 1 ; Wang, L. 1 ; Song, L. 1 ;
作者机构： 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
关键词： Aeromonas hydrophila infection; chinese mitten crab; insulin receptor; immune response
期刊名称：ISJ-INVERTEBRATE SURVIVAL JOURNAL （ 影响因子：1.115； 五年影响因子：1.254 ）
ISSN： 1824-307X
年卷期： 2021 年 18 卷
页码：
收录情况： SCI
摘要： Insulin plays important roles in metabolic homeostasis during environmental challenges. The insulin receptor is a key molecule to receive and transduce insulin signals. In the present study, a novel insulin receptor was identified from the Chinese mitten crab Eriocheir sinensis (designated as EsIR). The coding region of EsIR gene was 3573 bp in length and encoded 1190 amino acids with all the functional domains of mammal insulin receptors, including furin-like domain, receptor L domain, transmembrane domain, and tyrosine kinase domain. Phylogenetic analysis showed that the EsIR shared the closest evolutionary relationship with the insulin receptor from Macrobrachium rosenbergii. Cell transfection experiments confirmed that EsIR proteins were localized on the cytomembrane. The mRNA transcripts of EsIR were widely distributed in various tissues with higher abundance in hepatopancreas and eyestalk of E. sinensis. After Aeromonas hydrophila stimulation, the expression level of EsIR mRNA decreased from 3 h to 6 h, and then increased at 12 h. The conserved structure and subcellular localization of EsIR together with its sensitivity to A. hydrophila stimulation implied that EsIR was probably involved in immune response of E. sinensis. The present study provided clues for the further investigation about the evolution and function of the insulin signaling pathway in invertebrates.
- 相关文献
作者其他论文 更多>>
-
ToxR modulates biofilm formation in fish pathogen Vibrio harveyi
作者：Zhang, Y.;Deng, Y.;Feng, J.;Hu, J.;Chen, H.;Guo, Z.;Su, Y.;Zhang, Y.;Hu, J.;Chen, H.;Gao, R.;Su, Y.
关键词：biofilm formation; gene knockout; phenotypic characterization; toxR; Vibrio harveyi
-
Effects of -ketoglutarate on the growth performance, amino acid metabolism and related gene expression of mirror carp (Cyprinus carpio)
作者：Wang, L.;Wei, Y.;Wang, C.;Li, J.;Zhao, Z.;Luo, L.;Du, X.;Qiyou, X.;Wei, Y.
关键词：amino acid;gene expression;glutamine;glutamine synthetase;mirror carp;-ketoglutarate
-
Effect of polychlorinated biphenyls on osmoregulatory response and apoptosis in GIFT tilapia, Oreochromis niloticus
作者：Zheng, Y.;Qiu, L.;Fan, L.;Song, C.;Meng, S.;Chen, J.;Zheng, Y.;Qiu, L.;Fan, L.;Song, C.;Meng, S.;Chen, J.;Zheng, Y.;Chen, J.
关键词：Apoptosis;Oreochromis niloticus;Osmoregulatory responses;Polychlorinated biphenyls
-
A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus
作者：Yang, H. -L.;Qiu, L.;Liu, Q.;Wan, X. -Y.;Liu, S.;Zhu, L. -L.;Yang, B.;Zhang, Q. -L.;Huang, J.;Yang, H. -L.;Qiu, L.;Liu, Q.;Liu, S.;Zhu, L. -L.;Yang, B.;Zhang, Q. -L.;Huang, J.
关键词：loop-mediated isothermal amplification; new genotype; polymerase chain reaction; shrimp; yellow head virus
-
Mass mortality caused by Cyprinid Herpesvirus 2 (CyHV-2) in Prussian carp (Carassius gibelio) in China
作者：He, J.;Zheng, X.;Jia, P.;Shi, X.;Lan, W.;Liu, H.;Wang, L.;Xie, J.;Xu, P.;Wang, L.;Liang, L.;Xie, J.;Xu, P.
关键词：
-
A preliminary genetic distinctness of four Coilia fishes (Clupeiformes : Engraulidae) inferred from mitochondrial DNA sequences
作者：Cheng, Q.;Cheng, H.;Wang, L.;Zhong, Y.;Lu, D.;Cheng, Q.
关键词：
-
Effect of weaning time on the performance of Trachinotus ovatus (Linnaeus 1758) larvae
作者：Ma, Z.;Zheng, P.;Guo, H.;Zhang, N.;Wang, L.;Jiang, S.;Zhang, D.;Ma, Z.;Qin, J. G.
关键词：early weaning;growth and survival;jaw malformation;mid-gut cell height;pompano Trachinotus ovatus;RNA/DNA ratio
