Assessment of Bacterial Community Composition and Dynamics in Alfalfa Silages With and Without Lactobacillus plantarum Inoculation Using Absolute Quantification 16S rRNA Sequencing
文献类型： 外文期刊
作者： Yang, Fengyuan 1 ; Zhao, Shanshan 1 ; Wang, Yuan 2 ; Fan, Xiaomiao 2 ; Wang, Yanping 1 ; Feng, Changsong 3 ;
作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Henan Prov Key Lab Ion Beam Bioengn, Zhengzhou, Peoples R China
2.Zhengzhou Univ, Coll Phys, Henan Prov Key Lab Ion Beam Bioengn, Zhengzhou, Peoples R China
3.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou, Peoples R China
关键词： absolute quantification 16S-seq; relative quantification 16S-seq; alfalfa silage; Lactobacillus plantarum; bacterial community
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2021 年 11 卷
页码：
收录情况： SCI
摘要： Relative quantification 16S-seq (RQS) has drawn deeper insights into bacterial community compositions in silage. However, it provides no information on dynamics of the total amount of bacterial DNA through the ensiling process and across different treatments. In this study, bacterial compositions in alfalfa silage with and without Lactobacillus plantarum inoculation after 10 and 60days of ensiling were investigated using absolute quantification 16S-seq (AQS), and bacterial composition and its interaction with fermentation properties of silage indicated by AQS and RQS were compared. Variation in total bacterial DNA amounts across different treatments and ensiling periods was illustrated by AQS. AQS indicated higher bacterial richness indices and closer correlations of these indices with fermentation properties than RQS via spearman's correlation analyses, as well as more taxa with significance on bacterial abundance via lefse analyses. In conclusion, AQS effectively illustrated the dynamics of bacterial communities during the ensiling process.
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