文献类型: 外文期刊
作者: Xie, Xiaoyong 1 ; Ye, Guoling 1 ; Bao, Yuyuan 1 ; Ying, Ziwei 1 ; Xie, Mujiao 4 ; Zhu, Changbo 1 ; Wang, Ruixuan 2 ;
作者机构: 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, Guangzhou, Peoples R China
2.Hanshan Normal Univ, Sch Life Sci & Food Engn, Chaozhou, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Peoples R China
关键词: amino acids; fatty acids; proteomics; Scortum barcoo; starvation
期刊名称:AQUACULTURE RESEARCH ( 影响因子:2.082; 五年影响因子:2.415 )
ISSN: 1355-557X
年卷期: 2021 年 52 卷 6 期
页码:
收录情况: SCI
摘要: Proteins related to antioxidant defence systems and energy maintenance play a vital role in resisting starvation in fish. However, studies on the underlying molecular mechanism of starvation stress in the jade perch Scortum barcoo are very limited. Thus, the proteomic changes in S. barcoo during fasting were investigated by stable isotope labelling and high-performance liquid chromatography-tandem mass spectrometry (iTRAQ-LC- MS/MS) in the present study. Therefore, a total of 3324 proteins were identified, including 134 that were significantly altered (p < 0.05, fold change >1.20 or <0.83) in the experimental groups compared with the control group. Among all the differentially expressed proteins, only protein-disulphide reductase (TXNDC12) was upregulated. One-third of the differentially expressed proteins were enriched in the amino acid metabolism pathway, tricarboxylic acid (TCA) cycle pathway, fatty acid metabolism pathway and oxidative phosphorylation pathway. Proteins differentially expressed in the experimental group, including AASS, OGDH, ACAD8, FAH, BHMT, AOC3, AOC2 and HIBADH, are components of pathways that control the metabolism of amino acids. The results suggested the involvement of antioxidant defence in the response to an oxidative imbalance, and GSH, SOD and AKP were regulated under conditions of starvation stress. In conclusion, the data indicated that starvation-induced changes in protein levels that might affect metabolism and the antioxidant system.
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