Establishment of a cell line from swim bladder of the Grass carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II
文献类型： 外文期刊
作者： Yang, Yuru 1 ; Wang, Yingying 1 ; Wang, Qing 1 ; Zeng, Weiwei 1 ; Li, Yingying 1 ; Yin, Jiyuan 1 ; Wu, Siyu 1 ; Shi, Cunb 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Aquat Anim Immune Technol Guangdong Prov, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou, Guangdong, Peoples R China
2.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
3.Foshan Univ, Guangdong Prov Key Lab Anim Mol Design & Precise, Key Lab Anim Mol Design & Precise Breeding, Guangdong Higher Educ Inst,Sch Life Sci & Engn, Foshan 440605, Peoples R China
关键词： Grass carp reovirus (GCRV); Fish cell line CiSB; GCRV Genotype II
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2021 年 151 卷
页码：
收录情况： SCI
摘要： A cell line was established from swim bladder of the Grass carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 degrees C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at passage 60 compared to the wild type Grass carp cells (2n = 48). Infection with GCRV II isolate Hunan1307 was tracked by immunofluorescence and virus titration assay. The virus titer reached 10(5.2) TCID50/mL on 7th days post infection (dpi). Healthy adult Grass carp that were challenged with the virus propagated onto CiSB cells, displayed the typical symptoms and histopathological changes of Grass carp hemorrhagic disease (GCHD). Therefore, the CiSB cells can be used to propagate GCRV II and serve as a useful tool to study the pathogenesis of GCHD.
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