文献类型： 外文期刊

作者： Sun, Q. ¹ ; Liu, Q. ¹ ; Di, R. ¹ ; Wang, Y. ¹ ; Gan, S. ² ; Liu, S. ² ; Wang, X. ¹ ; Hu, W. ¹ ; Cao, X. ¹ ; Pan, Zh ¹ ; Guo, X. ¹ ; Y ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Anim Genet & Breeding & Reprod, Beijing 100193, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China

3.Beijing Gen Stn Anim Husb, Beijing 100101, Peoples R China

4.Cairo Univ, Fac Agr, Dept Anim Prod, Giza 12613, Egypt

关键词： Altay sheep; Fat tail; THRSP gene; SNP

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2021 年 53 卷 2 期

页码：

收录情况： SCI

摘要： Sheep is a significant agricultural animal and food source for people worldwide. The fat tail/rump is considered as an adaptive selection under harsh challenges which serves as a fat store for the animal. Thyroid hormone responsive (THRSP) is a crucial protein for cellular de novo lipogenesis. THRSP gene encodes a nuclear protein which regulates fatty acid synthesis in lipogenic tissues. Identification of single nucleotide polymorphisms (SNPs) of sheep THRSP gene and their association with fat deposition were investigated using Altay and White Suffolk sheep. In addition, the messenger RNA expression profiling of THRSP in fat-tailed and thin-tailed breeds was compared among 8 different tissues. Four SNPs have been detected in position g. 205A C, c. 52C>T, c. 364A>T and c. 1031C>T of THRSP gene. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin tailed breed) by Sequenom MassArray. In the c.1031C>T locus, the frequency of T allele of fat-tail breed was significantly (p<0.001) higher than that of the thin-tailed breed using F test, which suggest that this locus may be associated with fat deposition in fat-tail breed. Gene expression levels in both subcutaneous adipose and tail fat tissues were significantly (p<0.01) higher than the other tissues analyzed. Levels of THRSP expression in liver, subcutaneous adipose and tail fat tissues in Tan sheep (thin-tailed breed) were significantly (p<0.01) higher than that in Altay sheep (fat-tailed breed). The results obtained might expand our understanding of the important regulating role of THRSP gene in lipogenesis in fat-tailed sheep breed.