Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X
文献类型： 外文期刊
作者： Hu, Shuzhen 1 ; Yin, Yueyan 2 ; Chen, Buyang 2 ; Lin, Qi 2 ; Tian, Yanzhen 2 ; Song, Xijiao 3 ; Peng, Jiejun 2 ; Zheng, H 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
2.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
3.Zhejiang Acad Agr Sci, Cent Lab, Hangzhou, Peoples R China
4.Yunnan Acad Tobacco Agr Sci, Kunming, Yunnan, Peoples R China
关键词： apoplast; coat protein; exosomes; potato virus X; viral particles
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）
ISSN： 1464-6722
年卷期： 2021 年 22 卷 4 期
页码：
收录情况： SCI
摘要： The apoplast is the extracellular space for signalling, nutrient transport, and plant-microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)-infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription-PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high-speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one-way manner and do not share the bidirectional transport of exosomes.
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