文献类型： 外文期刊

作者： Chen, Huatao ¹ ; Kumawat, Giriraj ¹ ; Yan, Yongliang ¹ ; Fan, Baojie ¹ ; Xu, Donghe ¹ ;

作者机构： 1.Japan Int Res Ctr Agr Sci JIRCAS, 1-1 Ohwashi, Tsukuba, Ibaraki 3058686, Japan

2.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

3.ICAR Indian Inst Soybean Res, Indore 452001, Madhya Pradesh, India

4.Xinjiang Acad Agr Sci, Inst Crop Germplasm Resources, Urumqi 830000, Xinjiang, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang 050035, Hebei, Peoples R China

关键词： Soybean; Primary root length; Quantitative trait loci (QTL); Residual heterozygous lines (RHLs); Near isogenic lines (NILs)

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background The root system provides nutrient absorption and is closely related to abiotic stress tolerance, but it is difficult to study the roots under field conditions. This study was conducted to identify quantitative trait loci (QTL) associated with primary root length (PRL) during soybean seedling growth in hydroponic conditions. A total of 103 F-7 recombinant inbred lines (RILs) derived from a cross between K099 (short primary root) and Fendou 16 (long primary root) were used to identify QTL for PRL in soybean. The RIL population was genotyped with 223 simple sequence repeats markers covering 20 chromosomes. Phenotyping for primary root length was performed for 3-weeks plants grown in hydoponic conditions. The identified QTL was validated in near isogenic lines and in a separate RIL population. Results QTL analysis using inclusive composite interval mapping method identified a major QTL on Gm16 between SSR markers Sat_165 and Satt621, explaining 30.25 % of the total phenotypic variation. The identified QTL, qRL16.1, was further confirmed in a segregating population derived from a residual heterozygous line (RHLs-98). To validate qRL16.1 in a different genetic background, QTL analysis was performed in another F-6 RIL population derived from a cross between Union (medium primary root) and Fendou 16, in which a major QTL was detected again in the same genomic region as qRL16.1, explaining 14 % of the total phenotypic variation for PRL. In addition, the effect of qRL16.1 was confirmed using two pair of near-isogenic lines (NILs). PRL was significantly higher in NILs possessing the qRL16.1 allele from Fendou 16 compared to allele from K099. Conclusions The qRL16.1 is a novel QTL for primary root length in soybean which provides important information on the genetic control of root development. Identification of this major QTL will facilitate positional cloning and DNA marker-assisted selection for root traits in soybean.