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Cell Culture-Derived Tilapia Lake Virus-Inactivated Vaccine Containing Montanide Adjuvant Provides High Protection against Viral Challenge for Tilapia

文献类型： 外文期刊

作者： Zeng, Weiwei 1 ; Wang, Yingying 2 ; Hu, Huzi 2 ; Wang, Qing 2 ; Bergmann, Sven M. 3 ; Wang, Yahui 2 ; Li, Bo 2 ; Lv, Yuef 1 ;

作者机构： 1.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan 528000, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Minist Agr,Key Lab Fishery Drug Dev, Guangzhou 510380, Peoples R China

3.Friedrich Loffler Inst FLI, Inst Infectol, Fed Res Inst Anim Hlth, D-17493 Greifswald, Germany

关键词： inactivated vaccine; tilapia lake virus; adjuvant; protection

期刊名称：VACCINES （ 影响因子：4.422； 五年影响因子：5.513 ）

ISSN：

年卷期： 2021 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Tilapia lake virus (TiLV) is a newly emerging pathogen responsible for high mortality and economic losses in the global tilapia industry. Currently, no antiviral therapy or vaccines are available for the control of this disease. The goal of the present study was to evaluate the immunological effects and protective efficacy of formaldehyde- and beta-propiolactone-inactivated vaccines against TiLV in the presence and absence of the Montanide IMS 1312 VG adjuvant in tilapia. We found that beta-propiolactone inactivation of viral particles generated a vaccine with a higher protection efficacy against virus challenge than did formaldehyde. The relative percent survivals of vaccinated fish at doses of 10(8), 10(7), and 10(6) 50% tissue culture infectious dose (TCID50)/mL were 42.9%, 28.5%, and 14.3% in the absence of the adjuvant and 85.7%, 64.3%, and 32.1% in its presence, respectively. The vaccine generated specific IgM and neutralizing antibodies against TiLV at 3 weeks following immunization that were significantly increased after a second booster immunization. The steady state mRNA levels of the genes tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interferon gamma (IFN-gamma), cluster of differentiation 4 (CD4), major histocompatibility complex (MHC)-Ia, and MHC-II were all increased and indicated successful immune stimulation against TiLV. The vaccine also significantly lowered the viral loads and resulted in significant increases in survival, indicating that the vaccine may also inhibit viral proliferation as well as stimulate a protective antibody response. The beta-propiolactone-inactivated TiLV vaccine coupled with the adjuvant Montanide IMS 1312 VG and booster immunizations can provide a high level of protection from virus challenge in tilapia.

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