An electrochemiluminescence sensor of magnetic glycosyl-imprinted microspheres based on multi-probe signal amplification for CD44v6 determination
文献类型： 外文期刊
作者： Ma, Xionghui 1 ; Jiang, Zejun 3 ; Li, Jianping 1 ;
作者机构： 1.Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, Peoples R China
2.Chinese Acad Trop Agr Sci, Hainan Prov Key Lab Qual & Safety Trop Fruits & V, Anal & Test Ctr, Haikou 571101, Hainan, Peoples R China
3.Guilin Univ Technol, Coll Environm Sci & Engn, Guilin 541004, Peoples R China
关键词： Glycosyl-imprinting sensor; CD44v6; Electrochemiluminescence; Oligomeric hyaluronic acid; Magnetic particles; Boronate affinity
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）
ISSN：
年卷期： 2021 年 333 卷
页码：
收录情况： SCI
摘要： A CD44v6 detection method was developed by using glycosyl-imprinted technique and enhanced with multi-probe signal amplification. Oligomeric hyaluronic acid was used as the template molecule, which was prepared by the cleavage of polyhyaluronic acid using hyaluronidase. Mercaptophenylboronic acid was self-assembled on the surface of core-shell Au@Fe3O4 magnetic microspheres through the covalent interaction between its thio group and Au. Then oligomeric hyaluronic acid (<10 kDa) was linked on Au@Fe3O4 via the formation of a cyclic ester between boronic acid and the ortho-dihydroxy group of oligomeric hyaluronic acid. By using oligomeric hyaluronic acid as a template and p-aminophenylboronic acid (p-APBA) as the functional monomer, boronate affinity glycosyl-imprinted polymer films were prepared on Au@Fe3O4 by chemical polymerization. The magnetic glycosyl-imprinted polymer microspheres (MGIP) were attached on the surface of the magnetron-controlled glassy carbon electrode (MGCE) as the recognition component rebinding for the template, and CD44v6 was specifically recognized by the binding of the imprinted cavities to the oligomeric hyaluronic acid. The boronic acid functionalized tris-(2,2'-bipyridyl) ruthenium (II) (Ru(bpy)(3)-BO3) was used as a molecular probe to label the adsorbed oligomeric hyaluronic acid of CD44v6. With the aid of the multiply captured Ru (bpy)(3)(2+) probes on CD44v6 and the high luminescence efficiency of Ru(bpy)(3)(2+), the electrochemiluminescence response of the sensor exhibits a high sensitivity for CD44v6 determination with a detection limit of 0.08 ng/L. The method was applied in the assay of CD44v6 in serum samples.
- 相关文献
作者其他论文 更多>>
-
A particle counting immunosensor for the sensitive detection of ochratoxin A via click chemistry-mediated signal amplification
作者：Chen, Rui;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Dong, Yongzhen;Chen, Yiping;Wang, Shu;Zhang, Jiangjiang
关键词：SiO 2 @CuO 2 nanoparticles; Particle counter; Polystyrene microspheres; Click chemistry; Ochratoxin A
-
Integrating photoinduced gold nanoparticle formation inhibition and salt-assisted microextraction for colorimetric sensing of 2-mercaptobenzothiazole
作者：Su, Linjing;Xu, Jingyan;Yu, Bingfeng;Zhang, Zhi;Xiong, Yuhao;Ma, Xionghui
关键词：Photoinduced gold nanoparticle formation; Salt-assisted liquid-liquid microextraction; 2-Mercaptobenzothiazole; Colorimetric sensing
-
Electrodes Modified with N/S@Co-Doped Carbon Nanomaterials Prepared by Solution Self-Assembly for Electrochemical Determination of Dopamine
作者：Pang, Chaohai;Chen, Rui;Wu, Xuejin;Huang, Yanxia;Zhong, Liqi;Ma, Xionghui;Li, Shuhuai;Deng, Yan
关键词：Catalysis; Dopamine; Electrochemical sensor; Carbon-based nanomaterials
-
A novel framework-targeted affinity-based sandwich photoelectrochemical sensor for trace acrylamide determination
作者：Zhu, Wenqian;Cao, Wenwen;Yi, Zhongsheng;Li, Shuhuai;Ma, Xionghui;Li, Jianping;Chen, Rui;Zhang, Zhen;Pang, Chaohai;Li, Shuhuai;Ma, Xionghui
关键词：Photoelectrochemical sensor; Acrylamide; Covalent organic framework; Sandwich-type; Aptamer
-
Amplified electrochemical detection of sulfadiazine based on Cu-BTC-encapsulated FeNi dual-atom catalysts with improved catalytic efficiency
作者：Li, Shuhuai;Zhu, Yinian;Zhu, Zongqiang;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Chen, Rui
关键词：
-
Electrochemiluminescence immunosensor for thiamethoxam detection using NiFe-LDH-loaded Cu single-atom catalysts
作者：Chen, Rui;Pang, Chaohai;Ma, Xionghui;Wu, Xuejin;Liu, Chunhua;Yin, Guihao;Zhang, Zhenshan;Li, Shuhuai;Li, Shuhuai
关键词：Electrochemiluminescence sensors; Thiamethoxam; NiFe-layered double hydroxides (LDHs); Cu single atoms; Aptamers
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者：Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词：Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
