文献类型： 外文期刊

作者： Liu, Tianjia ¹ ; Li, Muzi ² ; Liu, Zhongchi ² ; Ai, Xiaoyan ¹ ; Li, Yongping ³ ;

作者机构： 1.Hubei Acad Agr Sci, Fruit & Tea Subctr, Hubei Innovat Ctr Agr Sci & Technol, Inst Fruit & Tea, Wuhan, Peoples R China

2.Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

3.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China

4.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）

ISSN： 2662-6810

年卷期： 2021 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Cultivated strawberry (Fragaria x ananassa) is an important fruit crop species whose fruits are enjoyed by many worldwide. An octoploid of hybrid origin, the complex genome of this species was recently sequenced, serving as a key reference genome for cultivated strawberry and related species of the Rosaceae family. The current annotation of the F. ananassa genome mainly relies on ab initio predictions and, to a lesser extent, transcriptome data. Here, we present the structure and functional reannotation of the F. ananassa genome based on one PacBio full-length RNA library and ninety-two Illumina RNA-Seq libraries. This improved annotation of the F. ananassa genome, v1.0.a2, comprises a total of 108,447 gene models, with 97.85% complete BUSCOs. The models of 19,174 genes were modified, 360 new genes were identified, and 11,044 genes were found to have alternatively spliced isoforms. Additionally, we constructed a strawberry genome database (SGD) for strawberry gene homolog searching and annotation downloading. Finally, the transcriptome of the receptacles and achenes of F. ananassa at four developmental stages were reanalyzed and qualified, and the expression profiles of all the genes in this annotation are also provided. Together, this study provides an updated annotation of the F. ananassa genome, which will facilitate genomic analyses across the Rosaceae family and gene functional studies in cultivated strawberry.