Cloning, functional characterization and expression analysis of nuclear factor erythroid-2-related factor-2 of golden pompano (Trachinotus ovatus) and its response to air-exposure
文献类型： 外文期刊
作者： Xie, Jiajun 1 ; Chen, Xu 3 ; Niu, Jin 1 ;
作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
2.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Peoples R China
3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr, Guangzhou 510300, Peoples R China
关键词： Trachinotus ovatus; Nrf2; Clone; Air-exposure; Nrf2-ARE
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）
ISSN： 2352-5134
年卷期： 2021 年 19 卷
页码：
收录情况： SCI
摘要： Nrf2 is an important component of Nrf2-ARE signaling pathway. However, information about Nrf2 in golden pompano (Trachinotus ovatus) is quiet limited. Thus, the full length of Nrf2 was cloned from T. ovatus in this study. The full-length cDNA sequence was 2376 bp in length and comprised of a 1848 bp ORF preceded by 123 bp 5 ' UTR, and followed by a 405 bp UTR. The putative amino acid sequence of the Nrf2 contained 615 residues, consisting of a conservative basic region leucin zipper. The N terminal portion of Nrf2 is rich in glutamic and aspartic acids and functioned as an acidic type of activation domain. The C terminus of the protein contained a region with heptad repeats of leucines. The phylogenetic tree showed that Seriola dumerili and T. ovatus were clustered as a branch, and combined with Mastacembelus armatus, Oreochromis niloticus and Takifugu rubripes. This branch of 5 species clustered with other branch of Oncorhynchus mykiss and Salmo salar. Nrf2 gene was predominantly expressed in liver and gut. The relative expression of Nrf2 in liver was significantly higher than that in brain, kidney, spleen and heart. After air-exposure, the expression level of HIF-1 alpha, keap1, Nrf2, HO-1, SOD and GSH-PX were significantly higher in air-exposure group than that in control group. This present study showed that Nrf2 plays a pivotal role in oxidative stress caused by air-exposure in T. ovatus, providing information about response to oxidative stress on the basis of the Nrf2-ARE pathway in T. ovatus.
- 相关文献
作者其他论文 更多>>
-
Examination of the effects of triacylglycerol lipase on astaxanthin conversion in the black tiger prawn Penaeus monodon
作者：Li, Yun-Dong;Yang, Yu-kai;Huang, Jian-Hua;Li, Xin-Tao;Yang, Li-Shi;Jiang, Shi-Gui;Jiang, Song;Zhou, Fa-Lin;Li, Yun-Dong;Shi, Jian-Zhi;Yang, Qi-Bin;Li, Yun-Dong;Chen, Xu;Li, Xin-Tao
关键词：Adipokinetic hormone; Astaxanthin intake; Lipase; Pond background colour; Shrimp colour
-
Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimp Penaeus monodon under chronic low-salinity stress
作者：Li, Yun-Dong;Li, ErChao;Li, Yun-Dong;Si, Meng-Ru;Jiang, Shi-Gui;Jiang, Song;Yang, Li-Shi;Huang, Jian-Hua;Zhou, Fa-Lin;Li, Yun-Dong;Yang, Qi-Bin;Chen, Xu;Zhou, Fa-Lin;Li, Yun-Dong;Zhou, Fa-Lin;Li, ErChao
关键词：Penaeus Monodon; Chronic low-salinity stress; transcriptome; osmoregulation; adaptation mechanisms
-
Effect of Fly Maggot Protein as Dietary on Growth and Intestinal Microbial Community of Pacific White Shrimp Litopenaeus vannamei
作者：Li, Xintao;Yang, Lishi;Jiang, Shigui;Zhou, Falin;Jiang, Song;Li, Yundong;Duan, Yafei;Li, Xintao;Li, Yundong;Huang, Jianhua;Li, Yundong;Chen, Xu;Yang, Qibin
关键词：Litopenaeus vannamei; fly maggot protein; intestinal microorganisms; growth performance; survival rate
-
Effects of Dietary Inositol Supplementation on Growth, Digestive Performance, Antioxidant Capacity, and Body Composition of Golden Pompano (Trachinotus ovatus)
作者：Chen, Xu;Wang, Jun;Chen, Xu;Wang, Jun;Zhao, Wei
关键词：Trachinotus ovatus; inositol; growth; digestive performance; antioxidant capacity
-
Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, Penaeus monodon
作者：Fan, Rui;Li, Yundong;Yang, Qibin;Jiang, Song;Huang, Jianhua;Yang, Lishi;Chen, Xu;Zhou, Falin;Jiang, Shigui;Fan, Rui;Yang, Qibin;Chen, Xu;Zhou, Falin
关键词：glutaredoxin; Penaeus monodon; oxidative stress; SNPs
-
A CSDE1/Unr gene from Penaeus monodon: Molecular characterization, expression and association with tolerance to low salt stress
作者：Si, Meng-Ru;Li, Yun-Dong;Jiang, Shi-Gui;Jiang, Song;Yang, Li-Shi;Huang, Jian-Hua;Zhou, Fa-Lin;Si, Meng-Ru;Li, Yun-Dong;Jiang, Shi-Gui;Yang, Qi-Bin;Chen, Xu;Zhou, Fa-Lin
关键词：Penaeus monodon; RNA -containing cold shock domain binding; protein E1 ( PmCSDE1 ); Immune and stress; RNA interference (RNAi); Single nucleotide polymorphism (SNP)
-
Unraveling the effects of sulfamethoxazole on the composition of gut microbiota and immune responses in Stichopus variegatus
作者：Tan, Chunming;Zhao, Wang;Wen, Weigeng;Chen, Xu;Ma, Zhenhua;Yu, Gang;Tan, Chunming;Zhao, Wang;Wen, Weigeng;Chen, Xu;Ma, Zhenhua;Yu, Gang;Tan, Chunming;Zhao, Wang;Wen, Weigeng;Chen, Xu;Ma, Zhenhua
关键词：Stichopus variegatus; antibiotic; high-throughput sequencing; intestinal microbiota; immune enzymes
