您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

GmGPDH12, a mitochondrial FAD-GPDH from soybean, increases salt and osmotic stress resistance by modulating redox state and respiration

文献类型： 外文期刊

作者： Zhao, Ying 1 ; Li, Xin 2 ; Zhang, Zexin 1 ; Pan, Wenjing 1 ; Li, Sinan 1 ; Xing, Yun 1 ; Xin, Wanying 1 ; Zhang, Zhanguo; 1 ;

作者机构： 1.Northeast Agr Univ, Coll Agr, Harbin 150030, Heilongjiang, Peoples R China

2.Heilongjiang Acad Agr Sci, Key Lab Maize Genet & Breeding, Harbin 150030, Heilongjiang, Peoples R China

3.Heilongjiang Bayi Agr Univ, Coll Agr, Daqing 163319, Heilongjiang, Peoples R China

关键词： Redox homeostasis; Respiration characteristics; GmGPDH12; Salt stress; Osmotic stress

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2021 年 9 卷 1 期

页码：

收录情况： SCI

摘要： In plants, glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the interconversion of glycerol-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP) coupled to the reduction/oxidation of the nicotinamide adenine dinucleotide (NADH) pool, and plays a central role in glycerolipid metabolism and stress response. Previous studies have focused mainly on the NAD(+)-dependent GPDH isoforms, neglecting the role of flavin adenine dinucleotide (FAD)-dependent GPDHs. We isolated and characterized three mitochondrial-targeted FAD-GPDHs in soybean, of which one isoform (GmGPDH12) showed a significant transcriptional response to NaCl and mannitol treatments, suggesting the existence of a major FAD-GPDH isoform acting in soybean responses to salt and osmotic stress. An enzyme kinetic assay showed that the purified GmGPDH12 protein possessed the capacity to oxidize G3P to DHAP in the presence of FAD. Overexpression and RNA interference of GmGPDH12 in soybean hairy roots resulted in elevated tolerance and sensitivity to salt and osmotic stress, respectively. G3P contents were significantly lower in GmGPDH12-overexpressing hair roots and higher in knockdown hair roots, indicating that GmGPDH12 was essential for G3P catabolism. A significant perturbation in redox status of NADH, ascorbic acid (ASA) and glutathione (GSH) pools was observed in GmGPDH12-knockdown plants under stress conditions. The impaired redox balance was manifested by higher reactive oxygen species generation and consequent cell damage or death; however, overexpressing plants showed the opposite results for these traits. GmGPDH12 overexpression contributed to maintaining constant respiration rates under salt or osmotic stress by regulating mRNA levels of key mitochondrial respiratory enzymes. This study provides new evidence for the roles of mitochondria-localized GmGPDH12 in conferring resistance to salt or osmotic stress by maintaining cellular redox homeostasis, protecting cells and respiration from oxidative injury. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台