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Genetic and functional analysis of the Zn(II)(2)Cys(6) transcription factor HADA-1 in Hypsizygus marmoreus

文献类型： 外文期刊

作者： Zhang, Jinjing 1 ; Hao, Haibo 1 ; Liu, Hong 1 ; Wang, Qian 1 ; Chen, Mingjie 1 ; Feng, Zhiyong 1 ; Chen, Hui 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Res Ctr Edible Fungi Biotechnol & Engn, Key Lab Appl Mycol Resources & Utilizat, Minist Agr, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Inst Edible Fungi, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

3.Nanjing Agr Univ, Coll Life Sci, 1 Weigang Rd, Nanjing 210095, Peoples R China

关键词： Hypsizygus marmoreus; Zn(II)(2)Cys(6) transcription factor; Fruiting body development; Mitochondrial stability; Oxidative stress response; Carbon metabolism

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2021 年 105 卷 7 期

页码：

收录情况： SCI

摘要： Zn(II)(2)Cys(6) transcription factors are critical for the reproductive growth and sexual development of fungi, but their roles in Basidiomycota remain unclear. In this study, the Hypsizygus marmoreus gene hada-1 was shown to encode a Zn(II)(2)Cys(6) transcription factor, the growth rate of mycelia was decreased, hyphae were angulated, and fruiting body development was hindered in the hada-1-silenced strains. In addition, mitochondrial stability was lost, and the mitochondria morphologies changed from oval shaped to dumbbell or linear shaped in the silenced strains. Regarding mitochondrial instability, the mitochondrial complex II, III, and V activities and adenosine triphosphate content were significantly decreased. At the same time, the activities of the carbohydrate metabolism-related enzymes glucose-6-plosphatase, glucose dehydrogenase, and laccase were significantly decreased, which might have resulted in the reduction of carbon metabolism. Furthermore, hada-1 was shown to regulate the reactive oxygen species (ROS) level; compared with the wild-type (WT) strain, the silenced mycelia exhibited higher ROS contents and were more sensitive to oxidative stress. Taken together, these results indicate that, as a global regulator, hada-1 plays crucial roles in mycelial growth, fruiting body development, carbon metabolism, mitochondrial stability, and oxidative stress in the basidiomycete H. marmoreus.

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