文献类型: 外文期刊
作者: Han, Wu 1 ; Ji, Yun 1 ; Wu, Wei 1 ; Cheng, Jin-Kui 1 ; Feng, Han-Qian 1 ; Wang, Yi 1 ;
作者机构: 1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem SKLPPB, Beijing, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
3.China Agr Univ, Ctr Crop Funct Genom & Mol Breeding, Beijing, Peoples R China
关键词: Zea mays; ZmCIPK23; K+ uptake; K+ channel; ZMK1
期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:4.402; 五年影响因子:5.207 )
ISSN: 1664-462X
年卷期: 2021 年 12 卷
页码:
收录情况: SCI
摘要: Potassium (K+) is one of essential mineral elements for plant growth and development. K+ channels, especially AKT1-like channels, play crucial roles in K+ uptake in plant roots. Maize is one of important crops; however, the K+ uptake mechanism in maize is little known. Here, we report the physiological functions of K+ channel ZMK1 in K+ uptake and homeostasis in maize. ZMK1 is a homolog of Arabidopsis AKT1 channel in maize, and mainly expressed in maize root. Yeast complementation experiments and electrophysiological characterization in Xenopus oocytes indicated that ZMK1 could mediate K+ uptake. ZMK1 rescued the low-K+-sensitive phenotype of akt1 mutant and enhanced K+ uptake in Arabidopsis. Overexpression of ZMK1 also significantly increased K+ uptake activity in maize, but led to an oversensitive phenotype. Similar to AKT1 regulation, the protein kinase ZmCIPK23 interacted with ZMK1 and phosphorylated the cytosolic region of ZMK1, activating ZMK1-mediated K+ uptake. ZmCIPK23 could also complement the low-K+-sensitive phenotype of Arabidopsis cipk23/lks1 mutant. These findings demonstrate that ZMK1 together with ZmCIPK23 plays important roles in K+ uptake and homeostasis in maize.
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