您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genomic analyses and biological validation of candidate genes for rectal temperature as an indicator of heat stress in Holstein cattle

文献类型： 外文期刊

作者： Luo, Hanpeng 1 ; Li, Xiang 1 ; Hu, Lirong 1 ; Xu, Wei 1 ; Chu, Qin 2 ; Liu, Aoxing 1 ; Guo, Gang 4 ; Liu, Lin 5 ; Brito, Lui 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod, Natl Engn Lab Anim Breeding,Minist Agr China, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China

3.Aarhus Univ, Ctr Quantitat Genet & Genom, Dept Mol Biol & Genet, DK-8830 Tjele, Denmark

4.Beijing Sunlon Livestock Dev Co Ltd, Beijing 100029, Peoples R China

5.Beijing Dairy Cattle Ctr, Beijing 100192, Peoples R China

6.Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA

关键词： animal resilience; Chinese Holstein cattle; genome-wide association study; gene expression; heat stress

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：3.333； 五年影响因子：3.432 ）

ISSN： 0022-0302

年卷期： 2021 年 104 卷 4 期

页码：

收录情况： SCI

摘要： Heat stress is a major cause of welfare issues and economic losses to the worldwide dairy cattle industry. Genetic selection for heat tolerance has a great potential to positively affect the dairy industry, as the gains are permanent and cumulative over generations. Rectal temperature (RT) is hypothesized to be a good indicator trait of heat tolerance. Therefore, this study investigated the genetic architecture of RT by estimating genetic parameters, performing genome-wide association studies, and biologically validating potential candidate genes identified to be related to RT in Holstein cattle. A total of 33,013 RT records from 7,598 cows were used in this study. In addition, 1,114 cows were genotyped using the Illumina 150K Bovine BeadChip (Illumina, San Diego, CA). Rectal temperature measurements taken in the morning (AMRT) and in the afternoon (PMRT) are moderately heritable traits, with estimates of 0.09 +/- 0.02 and 0.04 +/- 0.01, respectively. These 2 traits are also highly genetically correlated (r = 0.90 +/- 0.08). A total of 10 SNPs (located on BTA3, BTA4, BTA8, BTA13, BTA14, and BTA29) were found to be significantly associated with AMRT and PMRT. Subsequently, gene expression analyses were performed to validate the key functional genes identified (SPAG17, FAM107B, TSNARE1, RALYL, and PHRF1). This was done through in vitro exposure of peripheral blood mononuclear cells (PBMC) to different temperatures (37 degrees C, 39 degrees C, and 42 degrees C). The relative mRNA expression of 2 genes, FAM107B and PHRF1, significantly changed between the control and heat stressed PBMC. In summary, RT is heritable, and enough genetic variability exists to enable genetic improvement of heat tolerance in Holstein cattle. Important genomic regions were identified and biologically validated; FAM107B and PHRF1 are the main candidate genes identified to influence heat stress response in dairy cattle.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台