文献类型： 外文期刊
作者： Qi, Nanshan 1 ; Liao, Shenquan 1 ; Li, Juan 1 ; Wu, Caiyan 1 ; Lv, Minna 1 ; Liu, Yunqiu 2 ; Mohiuddin, Mudassar 1 ; Lin, 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Minist Agr,Inst Anim Hlth,Key Lab Livestock Dis P, Guangdong Lab Lingnan Modern Agr,Maoming Branch, Guangzhou 510640, Guangdong, Peoples R China
2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
关键词： Eimeria tenella; autophagy; ATG8; clone and expression; characterization
期刊名称：REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA （ 影响因子：1.024； 五年影响因子：1.416 ）
ISSN： 0103-846X
年卷期： 2021 年 30 卷 1 期
页码：
收录情况： SCI
摘要： Autophagy plays an important role in maintaining cell homeostasis through degradation of denatured proteins and other biological macromolecules. In recent years, many researchers focus on mechanism of autophagy in apicomplexan parasites, but little was known about this process in avian coccidia. In our present study. The cloning, sequencing and characterization of autophagy-related gene (Etatg8) were investigated by quantitative real-time PCR (RT-qPCR), western blotting (WB), indirect immunofluorescence assays (IFAs) and transmission electron microscopy (TEM), respectively. The results have shown 375-bp ORF of Etatg8, encoding a protein of 124 amino acids in E. tenella, the protein structure and properties are similar to other apicomplexan parasites. RT-qPCR revealed Etatg8 gene expression during four developmental stages in E. tenella, but their transcriptional levels were significantly higher at the unsporulated oocysts stage. WB and IFA showed that EtATG8 was lipidated to bind the autophagosome membrane under starvation or rapamycin conditions, and aggregated in the cytoplasm of sporozoites and merozoites, however, the process of autophagosome membrane production can be inhibited by 3-methyladenine. In conclusion, we found that E. tenella has a conserved autophagy mechanism like other apicomplexan parasites, and EtATG8 can be used as a marker for future research on autophagy targeting avian coccidia.
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