文献类型： 外文期刊
作者： Qi, Nanshan 1 ; Liao, Shenquan 1 ; Li, Juan 1 ; Wu, Caiyan 1 ; Lv, Minna 1 ; Liu, Yunqiu 2 ; Mohiuddin, Mudassar 1 ; Lin, 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Minist Agr,Inst Anim Hlth,Key Lab Livestock Dis P, Guangdong Lab Lingnan Modern Agr,Maoming Branch, Guangzhou 510640, Guangdong, Peoples R China
2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
关键词： Eimeria tenella; autophagy; ATG8; clone and expression; characterization
期刊名称：REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA （ 影响因子：1.024； 五年影响因子：1.416 ）
ISSN： 0103-846X
年卷期： 2021 年 30 卷 1 期
页码：
收录情况： SCI
摘要： Autophagy plays an important role in maintaining cell homeostasis through degradation of denatured proteins and other biological macromolecules. In recent years, many researchers focus on mechanism of autophagy in apicomplexan parasites, but little was known about this process in avian coccidia. In our present study. The cloning, sequencing and characterization of autophagy-related gene (Etatg8) were investigated by quantitative real-time PCR (RT-qPCR), western blotting (WB), indirect immunofluorescence assays (IFAs) and transmission electron microscopy (TEM), respectively. The results have shown 375-bp ORF of Etatg8, encoding a protein of 124 amino acids in E. tenella, the protein structure and properties are similar to other apicomplexan parasites. RT-qPCR revealed Etatg8 gene expression during four developmental stages in E. tenella, but their transcriptional levels were significantly higher at the unsporulated oocysts stage. WB and IFA showed that EtATG8 was lipidated to bind the autophagosome membrane under starvation or rapamycin conditions, and aggregated in the cytoplasm of sporozoites and merozoites, however, the process of autophagosome membrane production can be inhibited by 3-methyladenine. In conclusion, we found that E. tenella has a conserved autophagy mechanism like other apicomplexan parasites, and EtATG8 can be used as a marker for future research on autophagy targeting avian coccidia.
- 相关文献
作者其他论文 更多>>
-
Structure-Based Virtual Screening of Potential Inhibitors Targeting the Prolyl-tRNA Synthetase (PRS) in Eimeria tenella: Insights from Molecular Docking, ADMET Studies, and Molecular Dynamics Simulations
作者：Cai, Haiming;Liao, Shenquan;Li, Juan;Lv, Minna;Lin, Xuhui;Song, Yongle;Chen, Xiangjie;Zhu, Yibin;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei
关键词：avian coccidiosis; prolyl-tRNA synthetase;
Eimeria tenella ; docking protocol; molecular dynamics simulations; ADMET -
Development of a single-cell cloning technique for isolation of Pentatrichomonas hominis: a promising tool for diagnosing Trichomonas spp. infections in the pig breeding industry
作者：Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Qi, Nanshan;Sun, Mingfei;Fang, Siyun;Wang, Dingai;Yan, Zhuanqiang;Shen, Hanqin
关键词：
Trichomonas spp.; Isolation method; Single-cell cloning -
Research note: The critical role of the interaction between Eimeria tenella invasion protein RON2 and host receptor annexin A2 in mediating parasite invasion
作者：Ni, Junli;Chen, Xiangjie;Sun, Yunyun;Zhu, Yibin;Cai, Haiming;Song, Yongle;Lv, Minna;Li, Juan;Liao, Shenquan;Qi, Nanshan;Sun, Mingfei;Zhang, Jianfei;Lin, Xuhui;Ni, Junli;Sun, Yunyun;Gu, Youfang;Liu, Xinchao
关键词：Avian coccidia; Eimeria tenella; RON2; Invasion; Protein; receptor interactions
-
Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China
作者：Lu, Pengyun;Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Lv, Minna;Lin, Xuhui;Song, Yongle;Chen, Xiangjie;Zhang, Jianfei;Li, Juan;Sun, Mingfei;Lu, Pengyun;Shen, Hanqin;Fang, Siyun;Wang, Dingai;Yan, Zhuanqiang
关键词：
Pentatrichomonas hominis ; Pig infection; Zoonotic potential; Prevalence; Genetic diversity -
Unraveling the pathogenic potential of the Pentatrichomonas hominis PHGD strain: impact on IPEC-J2 cell growth, adhesion, and gene expression
作者：Zhu, Yibin;Cai, Haiming;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Hu, Junjing;Song, Yongle;Chen, Xiangjie;Yin, Lijun;Zhang, Jianfei;Liao, Shenquan;Sun, Mingfei;Fang, Siyun;Yan, Zhuanqiang;Wang, Dingai;Shen, Hanqin
关键词：Pentatrichomonas hominis; IPEC-J2; cell viability; inflammatory response
-
Advancing horizons in vegetable cultivation: a journey from ageold practices to high-tech greenhouse cultivation-a review
作者：Ahmed, Nazir;Li, Juan;Chachar, Sadaruddin;Hayat, Faisal;Tu, Panfeng;Zhang, Baige;Deng, Lansheng;Bozdar, Bilquees;Jahan, Itrat;Talpur, Afifa;Gishkori, Muhammad Saleem;Chachar, Zaid
关键词：seed treatments; soilless cultures; greenhouse technologies; precision agriculture; integrated pest management; digital monitoring
-
Metabolic Blockade-Based Genome Mining of Sea Anemone-Associated Streptomyces sp. S1502 Identifies Atypical Angucyclines WS-5995 A-E: Isolation, Identification, Biosynthetic Investigation, and Bioactivities
作者：Wang, Yuyang;Zhou, Le;Ju, Jianhua;Ma, Junying;Wang, Yuyang;Ju, Jianhua;Ma, Junying;Pan, Xiaoting;Qi, Nanshan;Sun, Mingfei;Liao, Zhangjun;Zhang, Hua
关键词：metabolic blockade-based genome mining; Streptomyces sp. S1502; WS-5995; biosynthesis; anticoccidial activity
