文献类型： 外文期刊

作者： Lian, Tengxiang ¹ ; Huang, Yingyong ¹ ; Xie, Xianan ⁵ ; Huo, Xing ⁸ ; Shahid, Muhammad Qasim ¹ ; Tian, Lei ⁹ ; Lan, Ta ¹ ;

作者机构： 1.South China Agr Univ, Coll Agr, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou, Peoples R China

2.Fujian Agr & Forestry Univ, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Key Lab Appl Genet Univ Fujian Prov, Fuzhou, Fujian, Peoples R China

4.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Crop Breeding Design, Fuzhou, Fujian, Peoples R China

5.South China Agr Univ, Coll Forestry & Landscape Architecture, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Peoples R China

6.South China Agr Univ, Coll Forestry & Landscape Architecture, Lingnan Guangdong Lab Modern Agr, Guangzhou, Peoples R China

7.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou, Peoples R China

8.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China

9.Ningxia Univ, Coll Agr, Yinchuan, Ningxia, Peoples R China

关键词： Oryza sativa; SST variation; rhizosphere bacterial community; soil metabolites; salt tolerant

期刊名称：MSYSTEMS （ 影响因子：6.633； 五年影响因子：7.389 ）

ISSN： 2379-5077

年卷期： 2020 年 5 卷 6 期

页码：

收录情况： SCI

摘要： Some plant-specific resistance genes could affect rhizosphere microorganisms by regulating the release of root exudates. In a previous study, the SST (seedling salt tolerant) gene in rice (Oryza sativa) was identified, and loss of SST function resulted in better plant adaptation to salt stress. However, whether the rice SST variation could alleviate salt stress via regulating soil metabolites and microbiota in the rhizosphere is still unknown. Here, we used transgenic plants with SST edited in the Huanghuazhan (HHZ) and Zhonghua 11 (ZH11) cultivars by the CRISPR/Cas9 system and found that loss of SST function increased the accumulation of potassium and reduced the accumulation of sodium ions in rice plants. Using 16S rRNA gene amplicon high throughput sequencing, we found that the mutant material shifted the rhizobacterial assembly under salt-free stress. Importantly, under salt stress, the sst, FIFIZcas, and ZH11cas plants significantly changed the assembly of the rhizobacteria. Furthermore, the rice SST gene also affected the soil metabolites, which were closely related to the dynamics of rhizosphere microbial communities, and we further determined the relationship between the rhizosphere microbiota and soil metabolites. Overall, our results show the effects of the rice SST gene on the response to salt stress associated with the soil microbiota and metabolites in the rhizosphere. This study reveals a helpful linkage among the rice SST gene, soil metabolites, and rhizobacterial community assembly and also provides a theoretical basis for improving crop adaptation through soil microbial management practices. IMPORTANCE Soil salinization is one of the major environmental stresses limiting crop productivity. Crops in agricultural ecosystems have developed various strategies to adapt to salt stress. We used rice mutant and CRISPR edited lines to investigate the relationships among the Squamosa promoter Binding Protein box (SBP box) family gene (SST/OsSPL)0), soil metabolites, and the rhizosphere bacterial community. We found that during salt stress, there are significant differences in the rhizosphere bacterial community and soil metabolites between the plants with the SST gene and those without it. Our findings provide a useful paradigm for revealing the roles of key genes of plants in shaping rhizosphere microbiomes and their relationships with soil metabolites and offer new insights into strategies to enhance rice tolerance to high salt levels from microbial and ecological perspectives.