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CrfP, a fratricide protein, contributes to natural transformation in Streptococcussuis

文献类型： 外文期刊

作者： Zhu, Yinchu 1 ; Ma, Jiale 2 ; Zhang, Yue 2 ; Zhong, Xiaojun 2 ; Bai, Qiankun 2 ; Dong, Wenyang 2 ; Pan, Zihao 2 ; Liu, Gu 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

3.Minist Agr, Key Lab Anim Bacteriol, Nanjing 210095, Peoples R China

4.OIE Reference Lab Swine Streptococcosis, Nanjing 210095, Peoples R China

5.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450000, Peoples R China

关键词： S; suis; CrfP; Murein hydrolase; Competence; Virulence

期刊名称：VETERINARY RESEARCH （ 影响因子：3.357； 五年影响因子：3.432 ）

ISSN： 0928-4249

年卷期： 2021 年 52 卷 1 期

页码：

收录情况： SCI

摘要： Streptococcussuis (S.suis) is an important zoonotic pathogen that causes septicaemia, meningitis and streptococcal toxic shock-like syndrome in its host, and recent studies have shown that S.suis could be competent for natural genetic transformation. Transformation is an important mechanism for the horizontal transfer of DNA, but some elements that affect the transformation process need to be further explored. Upon entering the competent state, Streptococcus species stimulate the transcription of competence-related genes that are responsible for exogenous DNA binding, uptake and processing. In this study, we performed conserved promoter motif and qRT-PCR analyses and identified CrfP as a novel murein hydrolase that is widespread in S.suis and stimulated with a peptide pheromone in the competent state through a process controlled by ComX. A bioinformatics analysis revealed that CrfP consists of a CHAP hydrolase domain and two bacterial Src homology 3-binding (SH3b) domains. Further characterization showed that CrfP could be exported to extracellular bacterial cells and lytic S.suis strains of different serotypes, and this finding was verified by TEM and a turbidity assay. To investigate the potential effect of CrfP in vivo, a gene-deletion mutant (Delta crfP) was constructed. Instead of stopping the natural transformation process, the inactivation of CrfP clearly reduced the effective transformation rate. Overall, these findings provide evidence showing that CrfP is important for S.suis serovar 2 competence.

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