BSA-SEQ-BASED METHOD FOR LOCATING KEY GENETIC SEGMENTS OF PEDUNCLE LENGTH IN BREWING DWARF SORGHUM [SORGHUM BICOLOR (L.) MOENCH]
文献类型： 外文期刊
作者： Yang, G. D. 1 ; Li, J. H. 1 ; Hu, Z. Y. 1 ; Hao, Z. Y. 1 ; Sun, B. S. 1 ; Liu, C. S. 1 ; Wang, Q. 2 ; Meng, X. X. 2 ; Guo, W. 3 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Keshan Branch, Harbin 161005, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Crop Resources Inst, Harbin 150086, Heilongjiang, Peoples R China
3.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin, Peoples R China
关键词： sorghum; peduncle length; BSA-seq; SNP; InDel
期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.7； 五年影响因子：0.9 ）
ISSN： 1589-1623
年卷期： 2023 年 21 卷 5 期
页码：
收录情况： SCI
摘要： Sorghum peduncle length is one of the key agronomic traits in sorghum production and has a critical impact on sorghum mechanization for harvesting. In this study, an F2 segregating population was constructed with significantly different long-peduncle KY133B and short-peduncle KY123B as parents, and the sorghum peduncle length gene was preliminarily located using BSA-seq technology. The association analysis was performed by ED algorithm and SNP-index (or InDel-index) algorithm, and finally the sorghum peduncle length association region was targeted on chromosome 7 and 10. In this study, the BSA-seq technique was used to rapidly and efficiently locate key genetic segments of sorghum peduncle length, which laid the foundation for subsequent functional validation and molecular studies of peduncle length genes.
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