Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies
文献类型： 外文期刊
作者： Liu, Fengli 1 ; Cao, Yanxin 1 ; Yan, Maokai 1 ; Sun, Mengxu 1 ; Zhang, Qingshui 1 ; Wang, Jun 2 ; Fu, Guanghua 3 ; Liu, R 1 ;
作者机构： 1.China Agr Univ, Coll Vet Med, Key Lab Anim Epidemiol, Minist Agr, Beijing 100193, Peoples R China
2.Jiaxing Vocat & Tech Coll, Agr & Environm Branch, Jiaxing 314036, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China
关键词： duck enteritis virus; monoclonal antibody; colloidal gold immunochromatographic assay strip
期刊名称：PATHOGENS （ 影响因子：3.018； ）
ISSN：
年卷期： 2021 年 10 卷 3 期
页码：
收录情况： SCI
摘要： Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 x 10(3) TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.
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