您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Qi, Qian 1 ; Dong, Zhongdian 3 ; Zhang, Ning 3 ; Wang, Liang 4 ; Shao, Changwei 2 ; Xu, Wenteng 2 ;

作者机构： 1.Henan Univ Sci & Technol, Luoyang 471000, Peoples R China

2.Chinese Acad Fishery Sci CAFS, Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Guangdong Ocean Univ, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang 524088, Peoples R China

4.Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China

关键词： Cynoglossus semilaevis; dhh; Expression analysis; Gonadal differentiation

期刊名称：GENE EXPRESSION PATTERNS （ 影响因子：0.897； 五年影响因子：1.239 ）

ISSN： 1567-133X

年卷期： 2021 年 39 卷

页码：

收录情况： SCI

摘要： Desert hedgehog (dhh) is a gene that is crucial for spermatogenesis and Leydig cell differentiation, but little is known regarding its influence on gonadal differentiation and development in fish. To understand its function, we cloned and characterized the dhh gene from Cynoglossus semilaevis (csdhh). The full length csdhh cDNA was 2473 bp, including a 1386 bp open reading frame (ORF), a 475 bp 5?-UTR, and a 612 bp 3?-UTR, encoding a predicted protein of 461 amino acid residues. Phylogenetic analysis showed that the putative protein belongs to the hedgehog (HH) family, and contains typical HH-N and HH-C domains. Amino acid sequence analysis revealed that CsDhh shares many features with Dhh analogues in other teleost species. Real-time quantitative PCR showed that csdhh was detected in eight different tissues in male and female tongue sole. During early embryonic development, the relative expression of the csdhh was significantly higher in the neural stage than in other embryonic developmental stages (P < 0.05). csdhh was detected at 20 days after hatching (dah) and at the critical period of male gonadal differentiation (80?95 dah), the relative expression of the csdhh was significantly higher in the male gonads than the female gonads. In 5, 8, and 12 month old gonads, the relative expression of the csdhh was significantly higher in male and pseudo-male than in female fish. The in situ hybridization (ISH) results showed that the hybridization signal was strongly expressed in primary and secondary spermatocytes, spermatids, and sertoli cells of the 1-year-old fish testis, with only weak signal expression in the corresponding ovarian tissue. These results suggest that csdhh is highly conserved in evolution and plays an important role in spermatogenesis in males and pseudo-males.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台