Rapid and Visual Identification of Chlorophyllum molybdites With Loop-Mediated Isothermal Amplification Method
文献类型： 外文期刊
作者： Wang, Nan 1 ; Zhao, Zhiyong 2 ; Gao, Jie 1 ; Tian, Enjing 3 ; Yu, Wenjie 1 ; Li, Hui 1 ; Zhang, Juan 1 ; Xie, Ruibin 1 ; Zha 1 ;
作者机构： 1.Chinese Acad Agr Sci, Key Lab Agroprod Qual & Safety, Inst Qual Stand & Testing Technol Agroprod, Beijing, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai, Peoples R China
3.Jilin Agr Univ, Chinese Minist Educ Edible & Med Fungi, Engn Res Ctr, Inst Mycol, Jilin, Jilin, Peoples R China
关键词： poisonous mushroom; loop-mediated isothermal amplification; Chlorophyllum molybdites; ITS; on-site rapid detection
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.235； 五年影响因子：4.926 ）
ISSN： 1664-302X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Chlorophyllum molybdites is a kind of common poisonous mushroom in China that is widely distributed in different areas. Food poisoning caused by accidentally eating C. molybdites has become more frequent in recent years. In 2019, there were 55 food poisoning incidents caused by eating this mushroom in China. Mushroom poisoning continues to be a common health issue of global concern. When mushroom poisoning occurs, an effective, simple, and rapid detection method is required for accurate clinical treatment or forensic analysis. For the first time, we established a loop-mediated isothermal amplification (LAMP) assay for the visual detection of C. molybdites. A set of specific LAMP primers was designed, and the specificity was confirmed against 43 different mushroom species. The LAMP method could detect as low as 1 pg of genomic DNA. Boiled mushrooms and artificial gastric-digested mushroom samples were prepared to test the applicability of the method, and the results showed that as low as 1% C. molybdites in boiled and digested samples could be successfully detected. The LAMP method can also be completed within 45 min, and the reaction results could be directly observed based on a color change under daylight by the naked eye. Therefore, the LAMP assay established in this study provides an accurate, sensitive, rapid, and low-cost method for the detection of C. molybdites.
- 相关文献
作者其他论文 更多>>
-
Modeling Growth Kinetics of Escherichia coli and Background Microflora in Hydroponically Grown Lettuce
作者：You, Xiaoyan;Yang, Dongqun;Yang, Dongqun;Qu, Yang;Zhao, Xiaoyan;Suo, Yujuan;Guo, Mingming;Zhang, Yangping
关键词：growth model; hydroponically grown lettuce; Escherichia coli; background microflora
-
Formaldehyde levels and formaldehyde-exposure-related health risk levels of edible mushrooms cultivated in China
作者：Shao, Yi;Liu, Haiyan;Huang, Liujuan;Zhao, Xiaoyan;Zhao, Zhiyong;Yang, Xianli;Shao, Yi;Liu, Haiyan;Zhao, Xiaoyan;Zhao, Zhiyong;Yang, Xianli;Zhou, Changyan;Zhou, Changyan
关键词：Edible mushrooms; Formaldehyde prevalence; Naturally produced formaldehyde; Dietary risk assessment; Point assessment; Probability assessment; Chinese inhabitants
-
Detection of gyromitrin-induced mushroom poisoning by an isotope-coded derivatization strategy combined with UPLC-MS/MS and its application
作者：Fan, Tingting;He, Xiangwei;Hengchao, E.;Zhang, Yanmei;Li, Xiaobei;Li, Xujiao;Yang, Xianli;Zhou, Changyan;Zhao, Zhiyong;Zhao, Xiaoyan;Zhao, Zhiyong
关键词：Biomarker; Detection; Gyromitrin; Isotope-coded Derivatization; UPLC-MS/MS
-
Molecular networking and equivalently quantitative ion strategy for discovery and quantification of glucosinolates in cauliflower and broccoli by liquid chromatography tandem mass spectrometry
作者：Hengchao, E.;Peng, Shuting;Zhao, Zhiyong;Zhang, Yanmei;Li, Xiaobei;Yang, Xianli;Fan, Tingting;Zhao, Xiaoyan;Zhou, Changyan;Yao, Xueqin
关键词：Glucosinolates; Molecular networking; Equivalently quantitative ion method; Cauliflower and broccoli; LC-MS/MS
-
Multiplex TaqMan real-time PCR assay for high-throughput identification of highly toxic mushroom species-induced foodborne poisoning
作者：Zhao, Lanxin;Zhao, Xiaoyan;Fan, Tingting;Zhang, Yanmei;Li, Xiaobei;Zhao, Zhiyong;Zhou, Changyan;Zhao, Lanxin;Tian, Enjing;Wang, Chunling;Zhao, Zhiyong;Zhou, Changyan
关键词：Highly toxic mushroom species; Multiplex real-time PCR; Simultaneous identification; Foodborne poisoning
-
Partial enzymolysis affects the digestion of tamarind seed polysaccharides in vitro: Degradation accelerates and gut microbiota regulates
作者：Li, Xujiao;Zhao, Xiaoyan;Zhou, Changyan;Li, Xujiao;Chen, Yinan;Song, Lihua;Wu, Yan;Wang, Jiangmei;Song, Zibo
关键词：Tamarind seed polysaccharide; Molecular weight; Enzyme -hydrolysis; Digestive property; Gut microbiota
-
Comprehensive investigation on non-volatile and volatile flavor compounds in the Morchella sextelata and Morchella importuna by UPLC-MS/ MS and GC x GC-TOF-MS
作者：Zhang, Yanmei;Li, Xiaobei;Zhao, Zhiyong;Fan, Tingting;Dong, Hui;He, Xiangwei;Zhao, Xiaoyan;Zhou, Changyan;Tang, Lihua
关键词：Morel; VOCs; non-VOCs; Flavor differences; UPLC-MS/MS; GC x GC-TOF/MS
