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TRIM9 is involved in facilitating Vibrio parahaemolyticus infection by inhibition of relish pathway in Penaeus monodon

文献类型： 外文期刊

作者： Peng, Chao 1 ; Zhao, Chao 2 ; Wang, Pengfei 2 ; Yan, Lulu 2 ; Fan, Sigang 2 ; Qiu, Lihua 2 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Resources, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Coll Fisheries & Life Sci,Minist Educ, Shanghai 201306, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China

3.Sanya Trop Fisheries Res Inst, Sanya, Hainan, Peoples R China

4.Chinese Acad Fishery Sci, Key Lab Aquat Genom, Minist Agr & Rural Affairs, Beijing, Peoples R China

关键词： Penaeus monodon; TRIM9; Vibrio parahaemolyticus; Relish; Antimicrobial peptides

期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：3.641； 五年影响因子：3.353 ）

ISSN： 0161-5890

年卷期： 2021 年 133 卷

页码：

收录情况： SCI

摘要： Tripartite motif-containing 9 (TRIM9) has been demonstrated to exert important roles in regulation of innate immune signaling. In this study, a novel TRIM9 homolog was identified from Penaeus monodon (named PmTRIM9). The open reading frame (ORF) of PmTRIM9 was 2064 bp, which encoding a 687-amino-acid polypeptide. Following Vibrio parahaemolyticus challenge, the expression levels of PmTRIM9 mRNA were significantly down-regulated in tested tissues. RNA interference and recombinant protein injection experiments were performed to explore the function of PmTRIM9, and the results showed it could facilitate V. parahaemolyticus replication and lead P. monodon more vulnerable to V. parahaemolyticus challenge. The dual-luciferase reporter assay showed that PmTRIM9 possessed the ability to inhibit the promoter activity in HEK293 T cells. Silencing of PmTRIM9 could increase the expression of the major NF-kappa B transcription factor, PmRelish. Further studies showed that knockdown of PmRelish promoted the V. parahaemolyticus infection and decreased the expression of specific antimicrobial peptides (AMPs), including PmCRU5, PmCRU7, PmALF6, PmALF3, PmLYZ and PmPEN5. However, knockdown of PmTRIM9 increased expression levels of the same AMPs, but except for PmCRU5, indicating that PmTRIM9 may negatively regulate the PmRelish-mediated expression of AMPs. All these results suggest that PmTRIM9 was involved in facilitating V. parahaemolyticus infection by inhibition of Relish pathway in P. monodon.

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