文献类型： 外文期刊

作者： Cao, Mingming ¹ ; Li, Shuju ¹ ; Deng, Qiang ¹ ; Wang, Huizhe ² ; Yang, Ruihuan ² ;

作者机构： 1.Tianjin Kernel Cucumber Res Inst, State Key Lab Vegetable Germplasm Innovat, Tianjin Key Lab Vegetable Breeding Enterprise, Tianjin 300192, Peoples R China

2.Tianjin Acad Agr Sci, Inst Cucumber Res, Tianjin 300192, Peoples R China

关键词： Cucumber; Pre-harvest sprouting; QTL-seq; qPHS4; 1

期刊名称：BMC GENOMICS （ 影响因子：3.594； 五年影响因子：4.093 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundCucumber (Cucumis sativus L.) is cultivated worldwide, and it is essential to produce enough high-quality seeds to meet demand. Pre-harvest sprouting (PHS) in cucumber is a critical problem and causes serious damage to seed production and quality. Nevertheless, the genetic basis and molecular mechanisms underlying cucumber PHS remain unclear. QTL-seq is an efficient approach for rapid quantitative trait loci (QTL) identification that simultaneously takes advantage of bulked-segregant analysis (BSA) and whole-genome resequencing. In the present research, QTL-seq analysis was performed to identify QTLs associated with PHS in cucumber using an F-2 segregating population.ResultsTwo QTLs that spanned 7.3Mb on Chromosome 4 and 0.15Mb on Chromosome 5 were identified by QTL-seq and named qPHS4.1 and qPHS5.1, respectively. Subsequently, SNP and InDel markers selected from the candidate regions were used to refine the intervals using the extended F-2 populations grown in the 2016 and 2017 seasons. Finally, qPHS4.1 was narrowed to 0.53Mb on chromosome 4 flanked by the markers SNP-16 and SNP-24 and was found to explain 19-22% of the phenotypic variation in cucumber PHS. These results reveal that qPHS4.1 is a major-effect QTL associated with PHS in cucumber. Based on gene annotations and qRT-PCR expression analyses, Csa4G622760 and Csa4G622800 were proposed as the candidate genes.ConclusionsThese results provide novel insights into the genetic mechanism controlling PHS in cucumber and highlight the potential for marker-assisted selection of PHS resistance breeding.