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Zn2+-dependent enhancement of Atrazine biodegradation by Klebsiella variicola FH-1

文献类型： 外文期刊

作者： Zhang, Jinpeng 1 ; Wu, Xian 2 ; Zhang, Xianghui 3 ; Pan, Hongyu 3 ; Shearer, Julia E. S. 4 ; Zhang, Hao 1 ; Sun, Fengj 1 ;

作者机构： 1.Jilin Agr Univ, Coll Plant Protect, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Inst Plant Protect, Gongzhuling 136100, Peoples R China

3.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

4.Georgia Gwinnett Coll, Sch Sci & Technol, Lawrenceville, GA 30043 USA

关键词： Hydrolase; Flow cytometry; Gene knockout; Red homologous recombination; Bioactivity; Cell membrane permeability

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：9.038； 五年影响因子：8.512 ）

ISSN： 0304-3894

年卷期： 2021 年 411 卷

页码：

收录情况： SCI

摘要： Degradation efficiency of Atrazine by Klebsiella variicola FH-1 is improved by the addition of Zn2+. Both the chromosome and plasmid genomes of strain FH-1 were sequenced and annotated to identify genes involved in the degradation of Atrazine. Four open reading frames (ORFs) 1040, 2582, 3597, and 4043 encoding Zn2+dependent hydrolases were knocked out to verify their predicted functions in the degradation of Atrazine. In the presence of Zn2+, the biodegradation efficiency of Atrazine by knockout mutant ?ORF 3597 was 13.7% lower than that of wild type (WT) of strain FH-1 but still 9.4% higher than that of WT without Zn2+. These results indicated that ORF 3597 played a synergistic role but may not be the sole factor involved in the degradation of Atrazine. The enzymatic activities of pydC encoded by ORF 3597 were further characterized in the degradation of Atrazine. Results of fluorescence staining and flow cytometry analyses showed that the survival of bacterial cells and cell membrane permeability were increased in the presence of Zn2+ at different concentrations. Our study provided a scientific foundation for further investigation of the biological mechanisms of improving the degradation of Atrazine by strain FH-1 with the presence of Zn2+.

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