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Analysis of antibiotic resistance phenotypes and genes of Escherichia coli from healthy swine in Guizhou, China

文献类型： 外文期刊

作者： Yu, Bo 1 ; Zhang, Yanan 2 ; Yang, Li 2 ; Xu, Jinge 2 ; Bu, Shijin 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China

2.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang, Peoples R China

关键词： antimicrobial resistance; extended-spectrum beta-lactamase; ESBLs; E. coli from swine; plasmid-mediated quinolone resistance; PMQR; resistance genes

期刊名称：ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH （ 影响因子：0.979； 五年影响因子：1.112 ）

ISSN： 0030-2465

年卷期： 2021 年 88 卷 1 期

页码：

收录情况： SCI

摘要： This study was carried out to investigate the resistance phenotypes and resistance genes of Escherichia coli from swine in Guizhou, China. A total of 47 E. coli strains isolated between 2013 and 2018 were tested using the Kirby-Bauer (K-B) method to verify their resistance to 19 common clinical antimicrobials. Five classes consisting of 29 resistance genes were detected using polymerase chain reaction. The status regarding extended-spectrum beta-lactamase (ESBL) and the relationship between ESBL CTX-M-type beta-lactamase genes and plasmid-mediated quinolone resistance (PMQR) genes were analysed. A total of 46 strains (97.9%) were found to be multidrug resistant. Amongst them, 27 strains (57.4%) were resistant to more than eight antimicrobials, and the maximum number of resistant antimicrobial agents was 16. Twenty antibiotic resistance genes were detected, including six beta-lactamase genes blaTEM (74.5%), blaCTX-M-9G (29.8%), blaDHA (17.0%), blaCTX-M-1G (10.6%), blaSHV (8.5%), blaOXA (2.1%), five aminoglycoside-modifying enzyme genes aac(3')-IV (93.6%), aadA1 (78.7%), aadA2 (76.6%), aac(3')-II c (55.3%), aac(6')-Ib (2.1%) and five amphenicol resistance genes floR (70.2%), cmlA (53.2%), cat2 (10.6%), cat1 (6.4%), cmlB (2.1%), three PMQR genes qnrS (55.3%), oqxA (53.2%), qepA (27.7%) and polypeptide resistance gene mcr-1 (40.4%). The detection rate of ESBL-positive strains was 80.9% (38/47) and ESBL TEM-type was the most abundant ESBLs. The percentage of the PMQR gene in blaCTX-M-positive strains was high, and the detection rate of blaCTX-M-9G was the highest in CTX-M type. It is clear that multiple drug resistant E. coli is common in healthy swine in this study. Extended-spectrum beta-lactamase is very abundant in the E. coli strains isolated from swine and most of them are multiple compound genotypes.

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