文献类型： 外文期刊

作者： Yu, Chunwei ¹ ; Chen, Hui ¹ ; Du, Donghua ¹ ; Lv, Wenting ¹ ; Li, Songjian ¹ ; Li, Dongfang ¹ ; Xu, Zixuan ¹ ; Gao, Min ² ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Vet Med, Hohhot 010018, Peoples R China

2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010018, Peoples R China

关键词： β -Glucan; Oxidative stress; Dectin-1; Nrf2; HO-1

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：2.892； 五年影响因子：2.883 ）

ISSN： 1355-8145

年卷期：

页码：

收录情况： SCI

摘要： beta-Glucan from Saccharomyces cerevisiae has been described to be effective antioxidants, but the specific antioxidation mechanism of beta-glucan is unclear. The objectives of this research were to determine whether the beta-glucan from Saccharomyces cerevisiae could regulate oxidative stress through the Dectin-1/Nrf2/HO-1 signaling pathway in lipopolysaccharides (LPS)-stimulated RAW264.7 cells. In this study, we examined the effects of beta-glucan on the enzyme activity or production of oxidative stress indicators in LPS-stimulated RAW264.7 cells by biochemical analysis and the protein expression of key factors of Dectin-1/Nrf2/HO-1 signaling pathway by immunofluorescence and western blot. The biochemical analysis results showed that beta-glucan increased the LPS-induced downregulation of enzyme activity of intracellular heme oxygenase (HO), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while decreasing the production of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, immunofluorescence results showed that beta-glucan can activate the nuclear factor erythroid 2-related factor 2 (Nrf2). The antioxidant mechanism study indicated that beta-glucan activated dendritic-cell-associated C-type lectin 1 (Dectin-1) receptors mediated Nrf2/HO-1 signaling pathway, thereby downregulating the production of ROS and thus produced the antioxidant effects in LPS-stimulated RAW 264.7 cells. In conclusion, these results indicate that beta-glucan potently alleviated oxidative stress via Dectin-1/Nrf2/HO-1 in LPS-stimulated RAW 264.7 cells.