A microfluidic colorimetric biosensor for in-field detection of Salmonella in fresh-cut vegetables using thiolated polystyrene microspheres, hose-based microvalve and smartphone imaging APP
文献类型： 外文期刊
作者： Man, Yan 1 ; Ban, Meijing 4 ; Li, An 1 ; Jin, Xinxin 1 ; Du, Yuanfang 1 ; Pan, Ligang 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
2.Minist Agr PR China, Risk Assessment Lab Agroprod Beijing, Beijing 100097, Peoples R China
3.Beijing Municipal Key Lab Agr Environm Monitoring, Beijing 100097, Peoples R China
4.Beijing Univ Agr, Sch Food Sci & Engn, Beijing 102206, Peoples R China
关键词： Microfluidic chip; Polystyrene microsphere; Colorimetric; Foodborne pathogen; Microvalve; Smartphone; Fresh-cut vegetable
期刊名称：FOOD CHEMISTRY （ 影响因子：6.306； 五年影响因子：6.219 ）
ISSN： 0308-8146
年卷期： 2021 年 354 卷
页码：
收录情况： SCI
摘要： A microfluidic colorimetric biosensor was developed using thiolated polystyrene microspheres (SH-PSs) for aggregating of gold nanoparticles (AuNPs), a novel hose-based microvalve for controlling the flow direction, and a smartphone imaging APP for monitoring colorimetric signals. Aptamer-PS-cysteamine conjugates were used as detection probes and reacted with Salmonella in samples. Complementary DNA - magnetic nanoparticle (cDNA MNP) conjugates were used as capture probes, reacted with the free aptamer-PS-cysteamine conjugates. AuNPs were aggregated on the surface of Salmonella-aptamer-PS-cysteamine conjugates, resulting in a visible color change in the detection chamber, which indicating different concentrations of Salmonella. The limit of detection was low to 6.0 ? 101 cfu/mL. The microfluidic biosensor exhibited a good specificity. It was evaluated by analyzing salad samples spiked with Salmonella. The recoveries ranged from 91.68% to 113.76%, which indicated its potential application in real samples.
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