A microfluidic colorimetric biosensor for in-field detection of Salmonella in fresh-cut vegetables using thiolated polystyrene microspheres, hose-based microvalve and smartphone imaging APP
文献类型： 外文期刊
作者： Man, Yan 1 ; Ban, Meijing 4 ; Li, An 1 ; Jin, Xinxin 1 ; Du, Yuanfang 1 ; Pan, Ligang 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
2.Minist Agr PR China, Risk Assessment Lab Agroprod Beijing, Beijing 100097, Peoples R China
3.Beijing Municipal Key Lab Agr Environm Monitoring, Beijing 100097, Peoples R China
4.Beijing Univ Agr, Sch Food Sci & Engn, Beijing 102206, Peoples R China
关键词： Microfluidic chip; Polystyrene microsphere; Colorimetric; Foodborne pathogen; Microvalve; Smartphone; Fresh-cut vegetable
期刊名称：FOOD CHEMISTRY （ 影响因子：6.306； 五年影响因子：6.219 ）
ISSN： 0308-8146
年卷期： 2021 年 354 卷
页码：
收录情况： SCI
摘要： A microfluidic colorimetric biosensor was developed using thiolated polystyrene microspheres (SH-PSs) for aggregating of gold nanoparticles (AuNPs), a novel hose-based microvalve for controlling the flow direction, and a smartphone imaging APP for monitoring colorimetric signals. Aptamer-PS-cysteamine conjugates were used as detection probes and reacted with Salmonella in samples. Complementary DNA - magnetic nanoparticle (cDNA MNP) conjugates were used as capture probes, reacted with the free aptamer-PS-cysteamine conjugates. AuNPs were aggregated on the surface of Salmonella-aptamer-PS-cysteamine conjugates, resulting in a visible color change in the detection chamber, which indicating different concentrations of Salmonella. The limit of detection was low to 6.0 ? 101 cfu/mL. The microfluidic biosensor exhibited a good specificity. It was evaluated by analyzing salad samples spiked with Salmonella. The recoveries ranged from 91.68% to 113.76%, which indicated its potential application in real samples.
- 相关文献
作者其他论文 更多>>
-
Application and evaluation of molecular docking for aptamer and small molecular interaction- A case study with tetracycline antibiotics
作者：Liang, Gang;Zhao, Jie;Pan, Ligang;Gong, Wenwen;Liang, Gang;Zhao, Jie;Pan, Ligang;Gong, Wenwen;Gao, Yufei;Xie, Tao;Zhen, Jianhui
关键词：Steady-state aptamers of tertiary structure; Molecular docking; Aptamer; Antibiotics
-
Magnetic Multi-Walled Carbon Nanotubes Nanomaterials as Magnetic Solid Phase Extraction Adsorbents for Simultaneous Determination of Multiple Mycotoxins in Agricultural Products by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Ma, Shuai;Liang, Gang;Man, Yan;Jin, Xin-Xin;Li, An
关键词：Mycotoxins; Magnetic multi-walled carbon nanotubes; Magnetic solid-phase extraction; Ultra-high performance liquid chromatography-tandem mass spectrometry
-
A new approach to detecting sugar syrup addition to honey: Stable isotope analysis of hexamethylenetetramine synthesised from honey monosaccharides (fructose and glucose)
作者：Li, An;Abrahim, Aiman;Islam, Marivil;Vlachou, Christina;Kelly, Simon D.;Li, An;Mejias, Enrique;Halim, Nur Hafizati Abdul;Frew, Russell
关键词：Honey; Authenticity; Hydrogen isotope; Carbon isotope; Hexamethylenetetramine; Hexamine; EA-IRMS
-
A microfluidic concentration gradient colorimetric system for rapid detection of nitrite in surface water
作者：Man, Yan;Tan, Huimin;Jin, Xinxin;Tao, Jing;Pan, Ligang;Man, Yan;Tan, Huimin;Jin, Xinxin;Tao, Jing;Pan, Ligang;Yu, Kaijia
关键词：Nitrite; Microfluidic chip; Colorimetric; Smartphone; Concentration gradient
-
An integrated distance-based microfluidic aptasensor for visual quantitative detection of Salmonella with sample-in-answer-out capability
作者：Man, Yan;Ban, Meijing;Jin, Xinxin;Li, An;Tao, Jing;Pan, Ligang;Man, Yan;Ban, Meijing;Jin, Xinxin;Li, An;Tao, Jing;Pan, Ligang
关键词：Microfluidic chip; Distance; Colorimetric; Salmonella; Aptamer
-
Recent advances in Chinese food authentication and origin verification using isotope ratio mass spectrometry
作者：Li, Chunlin;Nie, Jing;Rogers, Karyne M.;Yuan, Yuwei;Kang, Xuming;Li, An;Farag, Mohamed A.;Xiao, Jianbo;Liu, Cuiling;Rogers, Karyne M.
关键词：Authentication; Traceability; China; Food products; Organic; Stable isotopes
-
An enzyme-free, ultrasensitive strategy for simultaneous screening of the p-nitrophenyl substituent organophosphorus pesticides
作者：Sun, Pengyuan;Li, Bingru;Zhao, Jie;Jia, Wenshen;Pan, Ligang;Gong, Wenwen;Liang, Gang;Sun, Pengyuan;Li, Bingru;Zhao, Jie;Jia, Wenshen;Pan, Ligang;Gong, Wenwen;Liang, Gang;Zhen, Jianhui
关键词：Enzyme -free; P-nitrophenyl substituent organophosphorus; pesticides; LC-MS; MS; Indirect detection
