Tributyrin Plays an Important Role in Regulating the Growth and Health Status of Juvenile Blunt Snout Bream (Megalobrama amblycephala), as Evidenced by Pathological Examination
文献类型： 外文期刊
作者： Liang, Hualiang 1 ; Ji, Ke 2 ; Ge, Xianping 1 ; Xi, Bingwen 1 ; Ren, Mingchun 1 ; Chen, Xiaoru 3 ;
作者机构： 1.Chinese Acad Fishery Sci CAFS, Freshwater Fisheries Res Ctr FFRC, Key Lab Genet Breeding Aquat Anim & Aquaculture B, Wuxi, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
3.Tongwei Co Ltd, Chengdu, Peoples R China
4.Hlth Aquaculture Key Lab Sichuan Prov, Chengdu, Peoples R China
关键词： juvenile blunt snout bream; tributyrin; growth; antioxidant capacity; immune responses
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：5.085； 五年影响因子：5.733 ）
ISSN： 1664-3224
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： The present study aimed to assess the role of tributyrin (TB) in regulating the growth and health status of juvenile blunt snout bream (Megalobrama amblycephala) through an 8-week feeding experiment. Six groups were fed experimental diets with added TB percentages of 0% (control group), 0.03%, 0.06%, 0.09%, 0.12% and 0.15%. The present results showed that TB supplementation in feed had some positive impacts on FW, WG, FCR and SGR, and the best results were found in the 0.06% TB group (P). However, TB supplementation in feed had no significant effects on SR, CF, VSI or whole-body composition (P>0.05). TB supplementation in feed increased antioxidant capacity and immunological capacity and attenuated the inflammatory response by increasing the activity of T-SOD, GPx, CAT and the levels of anti-inflammatory cytokines (IL-10 and TGF-beta) and decreasing the levels of MDA and anti-inflammatory cytokines (TNF-alpha) (P). Furthermore, TB supplementation improved immunity by increasing the levels of immunoglobulins (IgM and IgG), C3 and IFN-gamma (P). Surprisingly, 0.06%-0.12% TB supplementation significantly increased the content of IL-1 beta (P). However, TB supplementation in feed had no significant effects on the plasma content of GSH, HSP70, IL-8 and the activity of T-AOC (P>0.05). The possible mechanism was that TB activated PI3K/Akt/Nrf2 and inhibits the NF-kappa B signaling pathway, further regulating the mRNA levels of key genes with antioxidant capacity and the inflammatory response; for example, it increased the mRNA levels of Nrf2, Cu/Zn-SOD, HO-1, CAT, Akt, PI3K, GPx, IL-10, and TGF-beta and decreased the mRNA levels of NF-kappa B and TNF-alpha (P). In addition, 0.06%-0.15% TB supplementation significantly increased the mRNA levels of IL-1 beta (P). TB supplementation in feed had no significant effects on the mRNA levels of HSP70, Mn-SOD and IL-8 (P>0.05). Evidence was presented that TB supplementation decreased the mortality rate caused by Aeromonas hydrophila challenge. In pathological examination, TB supplementation prevented hepatic and intestinal damage. Generally, TB supplementation improved the growth performance of juvenile blunt snout bream. Furthermore, TB supplementation activated PI3K/Akt/Nrf2 and inhibited the NF-kappa B signaling pathway, regulating health status and preventing hepatic and intestinal damage.
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass ( Micropterus salmoides) in response to carbonate alkalinity stress
作者：Hua, Jixiang;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Li, Yan;Dong, Yalun;Jiang, Bingjie;Xi, Bingwen;Qiang, Jun
关键词：Micropterus salmoides; Carbonate alkalinity stress; Tissue damage; Serum biological chemistry; RNA-seq
-
Effects of Seaweed Polysaccharide on the Growth and Physiological Health of Largemouth Bass, Micropterus salmoides
作者：Huang, Dongyu;Liang, Hualiang;Ren, Mingchun;Gu, Jiaze;Xue, Chunyu
关键词：seaweed polysaccharide; immunity; endoplasmic reticulum stress
-
A Study on the Dietary Yeast Polysaccharide Supplementation in Growth Performance, Antioxidant Capacity, and Immunity of Juvenile Largemouth Bass (Micropterus salmoides)
作者：Qin, Junjie;Ren, Mingchun;Liang, Hualiang;Mi, Haifeng;Zhang, Lu;Teng, Tao;Yin, Heng;Ren, Mingchun;Huang, Dongyu;Liang, Hualiang
关键词：juvenile largemouth bass; dietary supplementation; yeast polysaccharide; antioxidant capacity; immunity
-
The Significant Enhancing Effect of Vitamin B6-Fortified Feed on the Intestinal Digestive Efficiency, Immunity, and Antioxidant Defense Mechanisms of Juvenile Largemouth Bass (Micropterus salmoides)
作者：Zhang, Leimin;Gu, Jiaze;Liang, Hualiang;Ren, Mingchun;Huang, Dongyu;Liang, Hualiang;Ren, Mingchun
关键词：vitamin B-6; largemouth bass (Micropterus salmoides); intestine; digestion; immunity; antioxidant
-
Comparative analysis of Oreochromis niloticus responses to Chlorella vulgaris and Lactobacillus-fermented Azolla economic feed supplements in biofloc and green systems: An in silico evaluation of metabolic and physiological responses
作者：Aboseif, Ahmed M.;Flefil, Nasser S.;Taha, Mostafa K. S.;Aboseif, Ahmed M.;El-Dein, Asmaa Negm;Ren, Mingchun;Liang, Hualiang;Hussian, Abd-Ellatif M.
关键词：Oreochromis niloticus; Lactobacillus-fermented Azolla; Chlorella vulgaris; Biofloc system; In silico evaluation
-
Impacts of Substituting Fish Meal With Hydrolyzed Feather Meal on the Growth Performance, Immunity, and Antioxidant Capacity of Juvenile Largemouth Bass (Micropterus salmoides)
作者：Zhou, Yanhong;Liang, Hualiang;Ren, Mingchun;Liang, Hualiang;Ren, Mingchun;Huang, Dongyu;Gu, Jiaze
关键词：antioxidant capacity; growth performance; hydrolyzed feather meal; immune function; juvenile largemouth bass
-
Evaluation of dietary vitamin B6 requirement of juvenile largemouth bass (Micropterus salmoides) on the basis of growth performance, transaminase activity, and nutrient metabolism
作者：Zhang, Leimin;Gu, Jiaze;Liang, Hualiang;Ren, Mingchun;Huang, Dongyu;Liang, Hualiang;Ren, Mingchun
关键词：Vitamin B 6; Largemouth bass ( Micropterus salmoides ); Growth; Transaminase; Nutrient metabolism
