文献类型： 外文期刊
作者： Leng, Yue 1 ; Sun, Yonghai 1 ; Lv, Chengyu 2 ; Li, Zhuolin 2 ; Yuan, Cuiping 2 ; Zhang, Jie 2 ; Li, Tiezhu 1 ; Wang, Yong 1 ;
作者机构： 1.Jilin Univ, Coll Food Sci & Engn, Changchun 130062, Peoples R China
2.Jilin Acad Agr Sci, Inst Agrofood Technol, Changchun 130033, Jilin, Peoples R China
关键词： Glucocorticoid receptor; beta-sitosterol; transactivation; transrepression; dissociate; molecular docking
期刊名称：PROTEIN AND PEPTIDE LETTERS （ 影响因子：1.156； 五年影响因子：1.054 ）
ISSN： 0929-8665
年卷期： 2021 年 28 卷 3 期
页码：
收录情况： SCI
摘要： Background: Although glucocorticoids (GCs) are characterized as powerful agents to treat inflammatory afflictions, they are accompanied by metabolic side effects which limit their usage. beta-Sitosterol, as a minor component found in extraction of vegetable oil, was reported to have anti-inflammatory effects in RAW 264.7 cells. Objective: To test whether beta-sitosterol has an effect to dissociate transrepression from transactivation as a selective novel GR binder, this work evaluated the dissociated characteristics of beta-sitosterol. Methods: The probable binding interaction between beta-sitosterol and GR was explored by molecular docking. The GR transcriptional activity of beta-sitosterol was assessed in the reporter gene assay. The ability of beta-sitosterol to modulate the transactivation and transrepression of GR was evaluated by real-time quantitative PCR analysis. Results and Discussion: In the present study, beta-sitosterol treatment cannot induce GR-mediated transactivation. beta-Sitosterol exerted a potential to inhibited the expression of GR target transrepressed gene without activating the expression of GR transactivation dependent gene. Molecular docking demonstrated that beta-Sitosterol was able to bind the ligand binding domain of GR but unable to induce GR activation. Conclusion: This work offers evidence that beta-sitosterol may serve as a selective GR modulator.
- 相关文献
作者其他论文 更多>>
-
Intercropping outweighs straw incorporation driving community and functional diversity of microarthropods after 5 years of tillage practices
作者：Liu, Yuhang;Gao, Qiang;Sun, Xin;Wang, Bin;Sun, Xin;Liu, Yuhang;Wang, Yongjun;Yao, Fanyun;Wang, Bin;Sun, Xin;Wu, Haitao;Sun, Xin
关键词：Maize-peanut intercropping; Microarthropods; Straw incorporation; Sustainable agriculture
-
Psoralen and Isopsoralen Activate Nuclear Factor Erythroid 2-Related Factor 2 Through Interaction With Kelch-Like ECH-Associated Protein 1
作者：Lv, Chengyu;Wang, Song;Sun, Chang;Chen, Yihao;Wang, Chao;Yuan, Cuiping;Qin, Fengxian;Li, Tiezhu;Liu, Jing
关键词：antioxidant response element; isopsoralen; molecular docking; Nrf2; psoralen
-
Leveraging cover crop functional traits and nitrogen synchronization for yield-optimized waxy corn production systems
作者：Sun, Mengjing;Zhang, Long;Zhou, Jiangkuo;Liu, Ziping;Peng, Cong;Jia, Zechen;Lv, Yanjie;Wang, Yongjun;Sun, Mengjing;Zhang, Long;Zhou, Jiangkuo;Liu, Ziping;Peng, Cong;Jia, Zechen;Lv, Yanjie;Wang, Yongjun;Sun, Mengjing;Zhang, Long;Zhou, Jiangkuo;Liu, Ziping;Peng, Cong;Jia, Zechen;Lv, Yanjie;Wang, Yongjun
关键词：waxy corn; cover crop; yield; nitrogen use efficiency; path analysis
-
Integrating physiological, metabolome and transcriptome revealed the response of maize seeds to combined cold and high soil moisture stresses
作者：Meng, Xiangzeng;Wang, Lichun;Wang, Yongjun;Meng, Xiangzeng;Cao, Yujun;Lv, Yanjie;Wang, Lichun;Wang, Yongjun
关键词：
-
The effects of a combination of maize/peanut intercropping and residue return on soil microbial nutrient limitation in maize fields
作者：Yao, Fanyun;Cao, Yujun;Liang, Jie;Liu, Xiaodan;Liu, Zhiming;Lv, Yanjie;Wei, Wenwen;Xu, Wenhua;Wang, Yongjun;Qi, Wei;Wang, Yongjun;Yu, Yang;Li, Xiang;Feng, Jian
关键词：Soil nutrients; Microbial biomass; Extracellular enzymes; C:N:P stoichiometry; Nutrient limitation
-
Thriving in adversity: Understanding how maize seeds respond to the challenge of combined cold and high humidity stress
作者：Meng, Xiangzeng;Chen, Denglong;Wang, Yongjun;Wang, Lichun;Meng, Xiangzeng;Chen, Denglong;Lv, Yanjie;Xu, Wenhua;Wang, Yongjun;Wang, Lichun
关键词：Seed germination; Abiotic stress; Antioxidant enzyme; Metabolomic; Glycolysis
-
Elimination of Intraspecific Competition Does Not Improve Maize Leaf Physiological and Biochemical Responses to Topsoil Degradation
作者：Zhang, Shan;Jia, Zechen;Lv, Yanjie;Wang, Yongjun;Zhang, Shan;Guo, Zhongxiao;Lv, Yanjie;Wang, Yongjun;Zhang, Xiaolong;Liu, Kaichang
关键词：topsoil depth; maize planting density; intraspecific competition; nitrogen metabolism enzymes; photosynthesis enzymes; yield variability
