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Analysis of recently duplicated TYRP1 genes and their effect on the formation of black patches in Oujiang-color common carp (Cyprinus carpio var. color)

文献类型： 外文期刊

作者： Chen, H. 1 ; Wang, J. 1 ; Du, J. 1 ; Mandal, B. K. 1 ; Si, Zh 1 ; Xu, X. 1 ; Yang, H. 1 ; Wang, Ch 1 ;

作者机构： 1.Shanghai Ocean Univ, Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

3.Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Hydrobiol, Hangzhou 310021, Peoples R China

关键词： albino; CRISPR; Cas9; functional redundancy; gene duplication; melanophore

期刊名称：ANIMAL GENETICS （ 影响因子：2.841； 五年影响因子：2.438 ）

ISSN： 0268-9146

年卷期：

页码：

收录情况： SCI

摘要： Tyrp1 gene, as a member of the tyrosinase family, has undergone a recent duplication event during fourth-round whole genome duplication in common carp. In this research, three Tyrp1 genes were identified in Oujiang-color common carp (Cyprinus carpio var. color). The similar expression patterns and close phylogenetic relationship indicated that Tyrp1c is homologous to Tyrp1b and possibly originated from the ancient Tyrp1b. The rates of synonymous and non-synonymous substitution (K-a/K-s) in Tyrp1 across teleost phylogeny indicated that Tyrp1a is more likely to be in the process of purifying selection. The CRISPR/Cas9 system was used to disrupt the Tyrp1 genes in zebrafish and the WB (black patches on white skin) strain of Oujiang-color common carp. The Tyrp1 loss of function variants in zebrafish and WB carp showed severe melanin deficiency in the skin. Meanwhile, inactivation of a single Tyrp1 gene did not obstruct melanin synthesis, which proved that the functional redundancy of Tyrp1 genes existed in both zebrafish and Oujiang-color common carp. Among the mosaic individuals with Tyrp1 genes in disrupted-color common carp, various mutations in Tyrp1b gene induced gray or brown phenotypes, suggesting that it may be bifunctional in Oujiang-color common carp. In addition, the phenotype of WB variants was different from that of WW (whole white skin), suggesting that Tyrp1 genes were not the key factor that caused the difference between WB and WW.

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