Identification of a novel crustacyanin-like lipocalin in Penaeus monodon: Molecular cloning, tissue distribution and its functional studies in astaxanthin accumulation
文献类型： 外文期刊
作者： Zhao, Chao 1 ; Peng, Chao 1 ; Fan, Sigang 1 ; Wang, Pengfei 1 ; Yan, Lulu 1 ; Xie, Zhuofang 1 ; Qiu, Lihua 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisher Res Inst, Guangzhou, Guangdong, Peoples R China
2.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China
3.Minist Agr, CAFS, Key Lab Aquat Gen, Beijing 100141, Peoples R China
关键词： Penaeus monodon; PmCLL; Astaxanthin accumulation; Gene expression; Functional study
期刊名称：AQUACULTURE （ 影响因子：3.224； 五年影响因子：3.591 ）
ISSN： 0044-8486
年卷期： 2021 年 539 卷
页码：
收录情况： SCI
摘要： Though astaxanthin has drawn a lot of attention in the last couple of years because of their great antioxidative activity, the molecular mechanism of astaxanthin accumulation in shrimp is still unknown. In this study, a novel crustacyanin-like lipocalin gene that can participate in the accumulation of astaxanthin was isolated and cloned from Penaeus monodon (designated as PmCLL). Multiple alignments and evolutionary tree analysis indicated that PmCLL had the highest homology and the closest evolutionary relationship with the crustacyanin-like lipocalin from Penaeus vannamei. In order to clarify the function of PmCLL in astaxanthin accumulation, changes in astaxanthin content were detected after silenced or overexpressed PmCLL gene. Furthermore, the in vitro binding activity of the PmCLL recombinant protein and astaxanthin was tested using High Performance Liquid Chromatography (HPLC) method. In summary, the results obtained from the present study would help us understand the molecular mechanism of PmCLL involved in astaxanthin accumulation in P. monodon.
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