Development of a HPTLC method with iterative calibration approach for quantitative evaluation of kinsenoside content in different types of Anoectochilus roxburghii
文献类型： 外文期刊
作者： Chen, Ying 1 ; Tan, Chu Shan 4 ; Ng, Chiew Hoong 4 ; Yam, Mun Fei 2 ; Wu, Shuisheng 2 ; Sun, Zhirong 1 ;
作者机构： 1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 102488, Peoples R China
2.Fujian Univ Tradit Chinese Med, Coll Pharm, Fuzhou 350122, Fujian, Peoples R China
3.Fujian Acad Agr Sci, Res Ctr Med Plant, Inst Agr Bioresource, Fuzhou 350003, Fujian, Peoples R China
4.Univ Sains Malaysia, Sch Pharmaceut Sci, Minden 11800, Pulau Pinang, Malaysia
关键词： Anoectochilus roxburghii; HPTLC; Kinsenoside
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：3.594； 五年影响因子：3.273 ）
ISSN： 0026-265X
年卷期： 2021 年 165 卷
页码：
收录情况： SCI
摘要： Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) or more commonly known as Jin Xian Lian is a widely adapted medicinal herb in the Asian continent. However, till date there are limited rapid analytical method such as thin layer chromatography method that acts as standard for quantifying the active ingredients of A. roxburghii. In this research, a simple and rapid method high performance thin layer chromatography (HPTLC)-anisaldehydesulfuric acid derivative with iterative calibration method was developed and validated to quantify the non UV sensitive bioactive compound kinsenoside in wild, cultivated and tissue cultured A. roxburghii plants. The optimum mobile phase identified for kinsenoside in A. roxburghii was chloroform/methanol/water (45:30:4,%v/v/ v). It was also proved that HPTLC method for determination of kinsenoside showed good accuracy, precision, stability and repeatability, with wide working concentration range of 0.099?6.365 mg/mL. Intra-day and interday precision (% RSD) of the developed HPTLC method were 5.08?7.57% and 6.72?8.72%, respectively. The % RSD of iterative calibration method was 3.139%. Through this study, it can be concluded that the proposed HPTLC method with iterative calibration approach was successfully applied for quantitative analysis of kinsenoside in three types of A. roxburghii plants.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification, Molecular Characterization, and Expression Analysis of the HSP70 and HSP90 Gene Families in Thamnaconus septentrionalis
作者：Chen, Ying;Chen, Ying;Chang, Qing;Zhang, Ziyang;Wu, Dan;Bian, Li;Chen, Siqing;Fang, Qinmei
关键词：Thamnaconus septentrionalis; HSP70; HSP90; tissue; developmental stage; abiotic stress
-
Discrimination of Dioscorea species (Chinese yam) using FT-IR integrated with chemometric approach
作者：Chen, Jingying;Liu, Baocai;Chen, Ying;Zhang, Wujun;Zhao, Yunqing;Huang, Yingzhen;Yam, Mun Fei;Tew, Wan Yin;Ong, Peng Shun;Yan, Chong Seng;Loh, Hui Wei;Yam, Mun Fei;Chen, Ying;Yam, Mun Fei
关键词：Dioscorea; FT-IR; Chemometric; Classification; Principal Component Analysis; Discriminant analysis
-
A novel machine learning scheme for classification of medicinal herbs based on 2D-FTIR fingerprints
作者：Yoon, Tiem Leong;Yeap, Zhao Qin;Yeap, Zhao Qin;Yam, Mun Fei;Tan, Chu Shan;Chen, Ying;Chen, Jingying;Yam, Mun Fei
关键词：Machine learning; Herbal classification; 2D-FTIR fingerprinting
-
Application of FT-IR spectroscopy and chemometric technique for the identification of three different parts of Camellia nitidissima and discrimination of its authenticated product
作者：Tew, Wan Yin;Ying, Chen;Zhang, Wujun;Liu, Baocai;Chen, Jingying;Tew, Wan Yin;Yam, Mun Fei;Ying, Chen;Yoon, Tiem Leong;Yam, Mun Fei
关键词：golden camellia; Fourier transform infrared spectroscopy; second-derivative infrared spectra; two-dimensional correlation infrared spectra; principal component analysis; orthogonal partial least square discriminant analysis
-
Vasorelaxant effect of 5,7,4 '- Trihydroxyflavanone (Naringenin) via endothelium dependent, potassium and calcium channels in Sprague Dawley rats: Aortic ring model
作者：Tan, Chu Shan;Tew, Wan Yin;Yam, Mun Fei;Chen Jingying;Tan, Chu Shan;Tew, Wan Yin;Yam, Mun Fei
关键词：Naringenin; Flavonoids; Vasorelaxation; Aortic ring assay; Calcium and potassium channels
-
Comparison of FTIR spectrum with chemometric and machine learning classifying analysis for differentiating guan-mutong a nephrotoxic and carcinogenic traditional chinese medicine with chuan-mutong
作者：Tan, Chu Shan;Leow, Shin Yee;Yam, Mun Fei;Ying, Chen;Tan, Choo Jun;Yoon, Tiem Leong;Chen Jingying;Yam, Mun Fei
关键词：Mutong; Tri-step Fourier Transform Instruments (FT-IR); PLS-DA; Machine learning; PCA analysis; Herbal adulteration
