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Overexpression of MtRAV3 enhances osmotic and salt tolerance and inhibits growth of Medicago truncatula

文献类型： 外文期刊

作者： Wang, Shumin 1 ; Guo, Tao 3 ; Shen, Yixin 2 ; Wang, Zhen 1 ; Kang, Junmei 1 ; Zhang, Jiaju 1 ; Yi, Fengyan 4 ; Yang, Qing 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

2.Nanjing Agr Univ, Coll Agrograssland Sci, Nanjing 210095, Jiangsu, Peoples R China

3.Beijing Forestry Univ, Coll Grassland Sci, Beijing 100083, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010000, Peoples R China

关键词： RAV transcription Factor; Medicago truncatula; Osmotic and salt tolerance; Flowering time; Plant architecture

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 2019影响因子：3.72； 五年影响因子：3.966 ）

ISSN： 0981-9428

年卷期： 2021 年 163 卷

页码：

收录情况： SCI

摘要： Related to ABI3/VP1 (RAV) transcription factors play important roles in regulating plant growth and stress tolerance, which have been studied in many plant species, but have remained largely unidentified in legumes. To functionally characterize RAV in legumes, MtRAV3 from legume model plant Medicago truncatula was isolated and its function was investigated by overexpressing MtRAV3 in M. truncatula. Expression analysis demonstrated that MtRAV3 was markedly induced by NaCl and polyethylene glycol (PEG). MtRAV3 overexpression enhanced tolerance of transgenic M. truncatula to mannitol, drought and salt stresses, and induced the expression of adversity-related genes, including MtWRKY76, MtMYB61, cold-acclimation specific protein 31 (MtCAS31), alternative oxidase 1 (MtAOX1) and ethylene response factor 1 (MtERF1). There were lower relative electrolyte leakage and higher chlorophyll content of leaves in the MtRAV3 overexpression plants than in wild type plants under both salt and drought stress. MtRAV3 overexpression M. truncatula were featured by some phenotypes of dwarfing, late flowering, more branches, smaller flower and leaf organs. Further investigations showed that the expression levels of DWARF14 (MtD14), CAROTENOID CLEAVAGE DIOXYGENASES 7 (MtCCD7) and GA3-oxidase1 (MtGA3ox1), which related to dwarf and branch phenotype, were obviously reduced, as well as MtGA3ox1' (MTR_1g011580), GA20-oxidase1 (MtGA20ox1), MtGA20ox1' (MTR_1g102070) and GA20-oxidase2 (MtGA20ox2) involved in gibberellins (GAs) pathway. Overall, our results revealed that MtRAV3 exerted an important role in adversity response and plant growth, was a multifunctional gene in M. truncatula, which provided reference for genetic improvement of alfalfa (Medicago sativa).

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