文献类型： 外文期刊
作者： Michael Bergmann, Sven 1 ; Dabels, Juliane 2 ; Klafack, Sandro 3 ; Jin, Yeonwha 1 ; Lee, Pei-Yu 4 ; Hofmann, Arndt 1 ;
作者机构： 1.Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Greifswald, Germany
2.Univ Rostock, Aquaculture & Sea Ranching, Rostock, Germany
3.Univ Med Greifswald, Inst Med Biochem & Mol Biol, Greifswald, Germany
4.GeneReach Biotechnol Corp, Dept Res & Dev, Taichung, Taiwan
5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Peoples R China
6.Foshan Univ, Sch Life Sci & Engn, Key Lab Anim Mol Design & Precise Breeding Guangd, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China
7.Res Inst Freshwater Aquaculture & Fisheries, Bogor, Indonesia
8.West Pomeranian Univ Technol, Fac Food Sci & Fisheries, Dept Aquat Bioengn & Aquaculture, Szczecin, Poland
9.Univ Stirling, Inst Aquaculture, Sch Nat Sci, Aquat Vaccine Unit, Stirling, Scotland
关键词： antibodies; carp; rainbow trout sera; enzyme‐ linked immunosorbent assay (ELISA); koi herpesvirus isolates; neutralizing antibodies
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.318； 五年影响因子：2.135 ）
ISSN： 0140-7775
年卷期：
页码：
收录情况： SCI
摘要： Koi herpesvirus (KHV) is a highly contagious virus that causes KHV disease (KHVD) inducing high mortality in carp and koi (Cyprinus carpio L.). In the late stage, latency occurs with very low, often non-detectable virus concentrations, which represents a challenge for virus detection. After validation according to OIE recommendations, an antibody ELISA was established to recognize antibodies of C. carpio against KHV infection. In this study, the ELISA was modified to detect anti-KHV antibodies from a non-cyprinid fish. Experimentally infected rainbow trout (Oncorhynchus mykiss) were able to transmit KHV to naive carp at two different temperatures, demonstrating their potential as a reservoir host. At 20 degrees C, KHVD was induced in carp but not at 15 degrees C. Unexpectedly, rainbow trout developed humoral response against KHV at both temperatures. In contrast to carp, at 15 degrees C trout produced neutralizing antibodies but not at 20 degrees C. While antibodies obtained from infected carp sera reacted in a similar way against all KHV, antibodies from rainbow trout sera reacted differently to the same isolates by ELISA. The data show that even when non-cyprinid fish species are infected with KHV, they can produce antibodies that differ from those observed in carp.
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